Supcre G9 Colony Counting/Screening/Antibacterial Zone Measurement Combined Device

SupcreG9 is a research grade model newly launched by Xunshu Technology in 2017, with software and hardware * configuration, integrating three major functions: colony counting, antibacterial zone measurement, and strain screening.

The combination of a 4/3-inch ultra large area array CMOS sensor and a large aperture high-definition fixed focus lens ensures that tiny bacteria in the deep layers of the culture medium are not missed, and the outline of the antibacterial zone is clearer and sharper, ensuring the accuracy and reproducibility of image segmentation.

The software features a wide range of modules, including quick statistics, advanced statistical tools, grid filters, 3M test strips, typical bacterial screening, strain characteristic description, double circle analysis, and inhibition zone measurement...

The system also has data security, audit tracking, and information management functions toAnti tampering technology, intelligent reconstruction technology of testing environment testing process, watermark signature technologyAs the core, build a multi-level and mutually constrained security management guarantee system that meets the requirements of * for food and drug testing computer information systems.

Architecture design to ensure system security and data security

The four tiered system architecture of "system administrator, data administrator, operator, and reviewer" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information.

System administrator: responsible for creating and managing accounts and login passwords for all operators and auditors.

Data administrator: responsible for archive management, sealing, and computer database management of all test data.

Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies

Auditor: Responsible for reviewing the test reports submitted by operators.

Watermark signature, tamper proof, intelligent reconstruction of testing process - achieving effective audit tracking

① Multi user password login, electronic signature, computer-generated operation review date;

② All operational procedures, such as images, dilution ratios, statistical tools, parameters used, test values, correction situations, etc., are automatically recorded in the database by the computer and cannot be modified by the operator;

③ By reproducing the testing environment and process, the reviewer can trace all the operations of the operator.

Wide band illumination, suspended dark field of view

Adopting the classic design of fully enclosed, wide band illumination technology, it eliminates the phenomenon of light spots and halos formed by the refraction of environmental stray light in glass culture dishes.

360 degree flexible mixed light source illumination, highlighting the color and texture of bacterial colonies; The bottom light source adopts Crystal Sharp suspended dark field illumination, which clearly outlines the outline of bacterial colonies.

Sharp display of image details

Equipped with a 16 megapixel 4/3-inch ultra large array color CMOS sensor and a high-performance 16mm large aperture fixed focus lens, the image sharpness under suspended dark field illumination * can better distinguish subtle differences between small colonies and insoluble impurities, improving the accuracy of colony counting; The edge contour of the antibacterial zone is more delicate, ensuring the repeatability and uniformity of the potency determination data.

28 image processing algorithms to deal with various difficult images

Xunshu - an image segmentation method based on the level set active contour model, combines the level set method and the active contour model. In the process of minimizing the energy functional, the active contour continuously approaches the segmentation target until the evolution of the active contour line stops (the energy functional is minimized), and the segmentation is completed. The basic principle is to embed curves or surfaces into high-dimensional level set functions, and use a high-dimensional function to express the evolution process of low dimensional curves or surfaces. Supcre G9 integrates 28 image processing algorithms to achieve accurate segmentation and statistics of various difficult colony images.

Double circle analysis

Microbial plate screening is often manually judged by observing the size and depth of the "transparent circle, discoloration circle, growth circle, hydrolysis circle, phosphorus dissolution circle, oil discharge circle, antibacterial circle, calcium dissolution circle, and hemolysis circle" formed by mutant colonies with the naked eye. The Xunshu double circle analysis module provides quantitative analysis methods: by accurately measuring the outer circle diameter and colony diameter, the area ratio and diameter ratio of the two are automatically calculated. Automatically sort and locate the corresponding colonies based on the size of the ratio. Can be used for screening antibiotics, enzyme preparations, organic acid producing bacteria, surfactants, petroleum, and pesticide degrading bacteria.

Multi-color automatic clustering

When there is a significant difference in the color of colonies in the color medium, the color clustering accuracy can be set to automatically distinguish colony types and quantities based on color.

Using multi parameter classification recognition

If the petri dish is mixed with multiple bacteria and the color difference of the colonies is not obvious, multi parameter classification can be used for identification, such as small differences in colony color, size, and contour.

The example picture shows a mixed probiotic preparation of Bacillus licheniformis and Bacillus subtilis.

Specific bacterial identification

Specific bacterial recognition function: Simply click on a single colony with the mouse to instantly obtain the number of colonies of the same type, the location and morphological characteristics of each colony.

Example image shows genetic engineering recombinant screening (blue and white spots)

Virological research - plaque/phage count

The plaque is usually small and has little contrast with the background of the agar plate containing bacteria. The SupcreG9, under suspended dark field illumination conditions, utilizes a combination of high pixel, ultra large array camera and large aperture lens to enhance the sharpness and contrast of plaque images, achieving accurate segmentation and counting of adherent plaques.

Multi regional statistical tool - suitable for OPKA, SBA, porous plates

The Xunshu multi area statistical algorithm can easily achieve synchronized one click counting of any number of areas, suitable for conditioning phagocytic bactericidal tests (OPKA) and serum bactericidal tests (SBA), as well as porous plate analysis.

One click measurement of large bacterial colonies

The cross method is commonly used in the field of plant protection to study the growth rate of hyphae, the growth rate of fungi, the inhibition rate of hyphae growth, and indoor toxicity determination. Due to the spread, looseness, and irregular edge divergence of fungi, the measurement has large human errors and low efficiency.

The one click tool of Supcre G9 can be conveniently and accurately used for the plate confrontation method to analyze the inhibitory ability of antagonistic bacteria on pathogenic fungal hyphae

Automatic contour extraction, colony feature description

The digital description system for colony morphology not only standardizes the characteristic description of bacteria, yeast, actinomycetes, and fungi, but also directly imports precise measurement data of colonies into the database, such as automatically generating colony color descriptions based on three-dimensional color data of value points.

Paper method drug sensitivity analysis

The Kirby Bauer paper diffusion method experiment uses cotton swabs to coat the bottom layer of sensitive bacteria. After cultivation, most of the antibacterial zone edges are not smooth, have gaps, or form fuzzy antibacterial zones. The quasi circle approximation and three-point circle determination algorithm of Xunshu can achieve the measurement of such antibacterial zones.

Antibacterial activity analysis

The antibacterial zone formed by punching method is mostly in an ideal circular shape. The automatic detection algorithm of Xunshu (based on precise edge detection of the antibacterial zone contour) can quickly extract the antibacterial zone contour and measure the diameter.

Main functions and technical indicators

1、 Lighting system

1. Light source

Visible light: High brightness tri color LED structured light

254nm UV: used for cavity disinfection and UV mutagenesis

2. Light path and lighting control

Fully enclosed dark box: eliminating environmental stray light interference

Upper light source: scene style 360 ° flexible shadowless lighting

Lower light source: Crystal Sharp floating dark field illumination

Up light, down light, dual light, UV, freely switch

Color temperature adjustable (3500K-8500K), light intensity adjustable

2、 Digital imaging

High definition industrial fixed focus lens: 16mm large target high-definition fixed focus lens with a resolution of 150 lp/mm

Ultra large array CMOS camera: 4/3-inch color CMOS sensor, 16 million pixels C-Mount

3、 Colony analysis module

1. Basic colony counting function

Type of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet, porous plate

One click intelligent counting (6 modes): flat sensing mode, three-dimensional sensing mode, small colony priority, large colony priority, same color bacteria priority, culture medium removal mode

Whole dish colony count: Count the total number of colonies and display them classified by 25 sizes

Region selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics

Diameter classification statistics: Set the diameter range and count colonies of specific sizes

Mouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes

Colony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies

2. Advanced colony counting function

? Spiral colony statistics: automatically count spiral plates according to FDA standards, and support exponential mode, slow exponential mode, uniform mode, proportional mode, lawn mode, etc. Compatible with SBI in the United States and IUL in Spain. Support the entry and exit inspection and quarantine industry standard SN/T 2098-2008

Dynamic adjustment statistics: The statistical results can be dynamically adjusted and corrected to quickly obtain the statistical effect.

Deviation estimation statistics: suitable for situations with multiple and complex colony colors.

Level set multi model algorithm: search operation, obtain * image segmentation effect, adapt to background changes in the culture medium

Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics

Trans statistics: suitable for extremely complex colony types and uniform culture medium background

Statistics of high adhesion bacteria: suitable for segmentation calculation of multiple adhesion bacteria

Removal of miscellaneous bacteria and impurities: Automatic removal of miscellaneous bacteria and impurities based on differences in morphology, size, and color

3. Grid filter membrane and 3M test piece

Black solid line grid with one click statistics

3M total bacterial count test piece, 3M * test piece: one click statistics

3M Escherichia coli test strip, 3M Escherichia coli/Escherichia coli rapid test strip: one click statistics+manual selection

4. Typical bacterial screening

Single color classification statistics: Select specific bacterial colonies based on color accuracy, diffusion, colony size, and contour features

Multi color automatic clustering: automatically distinguish 24 different colored colonies based on color clustering accuracy

Multi color screening: screening 1-8 color colonies at once

Transparent circle characteristic analysis: suitable for antibacterial circle, hydrolysis circle, discoloration circle, calcium dissolution circle, hemolysis circle, oil discharge circle, phosphorus dissolution circle analysis

Double color circle automatic filtering

5. Description of colony characteristics

Bacteria and yeast: features such as color, size, shape, surface morphology, edge, gloss, transparency, etc., intelligently described and sorted

Mold and actinomycetes: features such as front color, back color, size, surface morphology, edges, texture, etc., intelligently described and sorted

6. Microbial limit analysis tool

Applicability test of culture medium (adapted to the Chinese Pharmacopoeia 2015 edition)

Control bacterial examination - colony morphology

7. Special analysis

Mold proof testing: quantitative analysis of mold proof level

Serial statistics: suitable for complex bacterial colonies with uneven background in the culture medium

Parallel statistics: suitable for porous plate, OPKA, SBA analysis

8. Advanced Tools

Grid Clearing: Eliminating background interference in the filter membrane grid

Manual counting correction: adding or deleting colonies

Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area

Background text elimination: automatically eliminates marker interference

Artificial adhesion segmentation: manually segmenting multiple adhesive colonies

Parameter automatic conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion

Text and graphic annotation: various drawing tools and embedded Chinese and English text

9. Calibration and Measurement

Instrument calibration: The instrument comes with built-in calibration and manual correction calibration

One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes

Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony

Manual precise measurement: length, angle, radian, area, arc, any curve

10. Image processing

Image adjustment: grayscale image and negative phase diagram conversion; Brightness, contrast, and saturation adjustment; RGB adjustment

Image enhancement: sharpening, adaptive enhancement

Image filtering: median filtering, high pass filtering, Gaussian filtering, low-pass filtering, queue filtering, high pass Gaussian filtering

Edge detection: Sobel operator, Robert operator, Laplace operator, vertical detection, horizontal detection

Morphological operations: corrosion, dilation, opening operation, closing operation

4、 Data Security and Management

1. The four tiered system architecture of "system, data, operation, and review" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information

System Administrator (Top Level): Responsible for creating and managing accounts and login passwords for all operators and auditors. Ensure account and data isolation between operators and between operators and auditors.

Data Administrator (Deputy Senior): Responsible for archive management of all test data and database management of computers. Seal all approved test reports or backup and export original images and test data to ensure data integrity and security.

Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies.

Reviewer: Responsible for reviewing the test reports submitted by operators. Verify the data input and processing process, but have no authority to modify it; Review and return the suspicious report, and request the operator to retest it; The report that has been approved will be archived, and neither the auditor nor the operator has the right to delete it again to ensure the originality and authenticity of the data.

2. Data storage and export

Mainly based on electronic data, record: sample source, number, dilution, petri dish image, recognition effect, count value, statistical tools used, parameter settings, correction status, to ensure complete record information.

Meet quality audit requirements, and the stored electronic data can be printed and output in PDF or Excel format

3. Watermark signature technology, tamper proof technology, and intelligent reconstruction technology for testing processes to achieve effective audit tracking

4. Anti tampering technology

Adopting multi-user login management, the names of all operators and auditors are automatically recorded in the operation process and test report by the system; All operation dates and review dates are automatically generated by the computer to avoid errors or forgeries.

The entire operation process, including colony images, dish size, sample dilution, statistical tools, parameters used, total bacterial count obtained from testing, self checked and corrected total bacterial count, etc., is automatically recorded in the database by the computer and cannot be modified by the operator, providing all real data for subsequent audits.

5. Watermark signature technology

The test report with "approved" will automatically generate electronic signatures for the operator and auditor's accounts, and an anti-counterfeit "approved" watermark will be printed on the report.

6. Intelligent refactoring technology for testing process

The "reviewer" opens the "waiting for review" test record, and the computer automatically restores the entire process and testing environment of the operator, including: images of the culture dish measured at that time, test results, dish size, sample dilution, statistical tools and parameters used, total bacterial count obtained from the test, correction status

By reproducing the testing environment and testing process, the reviewer can trace all the operations of the operator, verify the accuracy of the test results, and achieve the purpose of audit tracking.

5、 Antibacterial zone analysis module

1. Multi mode measurement technology for Szone inhibition zone

Automatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones

Quasi circular approximation: Based on the contour of the antibacterial zone, circular fitting approximation is suitable for edge rupture and non-standard circular antibacterial zones

Manual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges

2. Determination of antibiotic potency

One dose method potency testing: suitable for the United States Pharmacopeia

Two dose method, three dose method, and combined calculation: suitable for the Chinese Pharmacopoeia 2015 edition

Repetitive self check: relative error ≤ 0.01%, repeated measurement accuracy ≤ 0.002mm

Uniformity self check: relative error ≤ 0.1%

Measurement difference between stations ≤ 0.2%

3. Shubatan sensitive β - lactase test

Pure water verification: Generate inhibition zones based on (A), (B), and (D), D-C≧3， B-A≦3 ， The judgment system is established

Automatically detect the (A), (B), (C), and (D) inhibition zones of three parallel samples and import the data

Automatically calculate the average value of parallel experiments and intelligently distinguish the positive and negative results.

Automatic alert for invalid reports

6、 Instrument specifications and configuration

SupcreG9 colony counting/screening/inhibition zone measurement combination instrument host 1 unit

Bacterial colony analysis software, automatic inhibition zone measurement software, antibiotic potency determination software, sulbactam sensitive β - lactam enzyme testing software

Integrated computer: dual core four thread CPU/4G memory/1T hard drive/21.5 'high-definition screen, Windows 7 system or above