Fully automatic colony counter

icountThe series is the fourth generation intelligent colony counter launched by Xunshu Technology in 2013. The newly designed three color LED hybrid light source eliminates the blue bias problem of the previous generation of white LED lighting and can truly restore the color of bacterial colonies. The upper light source is distributed in a 360 degree surround, resulting in a more uniform field of view; The newly designed Crystal Sharp floating dark field has greatly improved the contrast of the dark field, making it more suitable for observing small bacterial colonies.
Icount30 is a high-end, expandable intelligent colony counter in the icount series,Built in 254nm ultraviolet disinfection module reduces the risk of infection by pathogenic microorganisms, and is equipped with more image segmentation algorithms, suitable for complex colony imaging. Among them, icount30D has specialized microbial limit analysis tools, which are more suitable for drug testing.

Tri color LED light source
Long life, low power consumption, environmentally friendly three color LED mixed light, restores the true color of bacterial colonies, and eliminates the problem of blue bias in white LED lighting imaging.

Fully enclosed lighting
Adopting fully enclosed, wide band lighting technology and ergonomic porthole door design, it isolates the interference of ambient light and eliminates the phenomenon of light spots and halos caused by stray light refracted in glass culture dishes.

photoelectric conversion
High fidelity lens, 8 million CMOS, delicately displays the morphology of bacterial colonies and the details of the edge of the antibacterial zone. Users can choose a 6mm wide-angle lens to meet the needs of 150mm large culture dishes and porous plates.

UV disinfection
Built in 254nm ultraviolet lamp can solve the pollution problem caused by the use of colony analyzer *,Reduce the risk of infection by pathogenic microorganisms,It can also meet the needs of UV mutagenesis.

Advanced Statistics
Provide 5 advanced statistical algorithms that can achieve accurate colony counting by adjusting relevant parameters, suitable for complex petri dish statistical situations.

grid background
Specially designed to solve colony counting problems with grid backgrounds, such as grid filter membranes and 3M test pieces, it achieves automatic counting of 3M bacterial count test pieces, 3M * test pieces, black solid line grid filter membranes, and semi-automatic counting of 3M E. coli test pieces (requiring manual correction by users based on E. coli characteristics).

Clear background text
There are two common situations in microbiological experiments: the experimenter marks various symbols on the bottom of the culture dish with a marker pen; Due to the uncleaned culture dish, there are patchy stains and marks left, which interfere with the automatic recognition of colony images. The background restoration tool developed by Xunshu only requires two simple steps to restore the culture dish image to its original state, thereby improving the accuracy of colony image segmentation.

One click rapid measurement
Due to the spread, looseness, and irregular edge divergence of most fungal colonies, there is a large human error in measurement, resulting in low efficiency. The "one click mold measurement" module of Xunshu only requires clicking once on the edge of the colony with a "magic wand" to instantly measure the area, circumference, length, and length of the large mold.

Microbial Limit Analysis Tool (icount30D)
Applicability check of culture medium
The suitability test of culture medium is to compare the growth status or characteristics of positive bacteria between the test medium and the control medium to evaluate whether the test medium meets the testing requirements. Judgment criteria: The ratio of the average number of colonies on the tested culture medium to the average number of colonies on the control culture medium should be greater than 70%, and the morphology and size of the colonies should be the same as those on the control culture medium. Xunshu provides a simple and efficient statistical tool for microbial limit analysis, suitable for solving the applicability of bacterial, fungal, and yeast counting culture media. With just a simple mouse click, colony statistics and comparative analysis of culture media can be completed, and the results can be exported to EXCEL.

Control bacterial examination
When establishing a microbial limit testing method for drugs, validation of the control bacteria testing method should be conducted to confirm that the method used is suitable for the control bacteria testing of the drug. Xunshu provides a systematic colony morphology description tool for the validation experiment of bacterial inspection methods, avoiding the diversity of description results caused by personal language habits. The software provides a comprehensive library of colony morphology feature description information, and users only need to select with the mouse to complete target colony extraction and feature description, and export the results to EXCEL.

Special Analysis (icount30F)
The standard specifies the testing method for the anti mold performance of plastics, and the mold area is generally estimated through visual observation and measurement with a vernier caliper. In order to accurately determine the anti mold performance of plastic products, Xunshu provides a rapid mold growth level assessment tool - anti mold detection. By automatically extracting mold and sample contours, relevant values can be accurately calculated.

data management
The system adopts an open experimental report printing method, which can export statistical information to EXCEL and edit the report format according to industry requirements.
To protect the authenticity and originality of experimental data, the system sets three passwords: program startup password, database password, and administrator password.

Fully automatic colony counterFunctional and technical indicators

1、 Lighting system
1. light source
At the bottomVisible light: High brightness tri color LED structured light
At the bottom254nmUV: Used for cavity disinfection and UV mutagenesis
2. Light Path and Lighting Control
At the bottomFully enclosed dark box: eliminating environmental stray light interference
At the bottomUpper light source: Scene style 360 ° flexible shadowless lighting
At the bottomLower light source: Crystal Sharp suspended dark field illumination
At the bottomUp light, down light, dual light, UV, freely switch,Adjustable light intensity

2、 Digital imaging
At the bottomStandard definition industrial fixed focus lens
At the bottom800Ten thousand pixel professional CMOS camera

3、 Colony analysis module
1. Basic colony counting function
At the bottomType of petri dish: pouring, coating, membrane filtration, 3M paper sheet
At the bottomOne click intelligent counting (6 modes)
At the bottomWhole dish colony count: Count the total number of colonies and display them classified by 25 sizes
At the bottomRegion selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics
At the bottomDiameter classification statistics: Set the diameter range and count specific sizes of colonies
At the bottomMouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes
At the bottomColony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies
2. Advanced colony counting function
At the bottomDynamic adjustment statistics
At the bottomDeviation estimation statistics
At the bottomLevel Set Multi Model Algorithm
At the bottomSpecific colony statistics
At the bottomTrans statistics
At the bottomRemoval of miscellaneous bacteria and impurities
3. Grid filter membrane and 3M test piece
At the bottomBlack solid line grid, one click statistics
At the bottom3MTotal bacterial count test piece, 3M * test piece: one click statistics
At the bottom3MColiform test strip, 3M Escherichia coli/coliform rapid test strip: one click statistics+manual selection
4. Microbial Limit Analysis Tool (icount 30D)
At the bottomApplicability check of culture medium
At the bottomControl bacterial examination-Colony morphology
5. Special Analysis (icount 30F)
At the bottomMold proof testing: quantitative analysis of mold proof level
6. Advanced Tools
At the bottomGrid cleaning: eliminate background interference in the filter membrane grid
At the bottomManual counting correction: adding or deleting colonies
At the bottomExclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area
At the bottomBackground text elimination: automatically eliminates marker interference
At the bottomArtificial adhesion segmentation: manually segmenting multiple adhesive colonies
At the bottomAutomatic parameter conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion
Ø Text and graphic annotations
7. Calibration and Measurement
At the bottomInstrument calibration: The instrument comes with built-in calibration and manual correction calibration
At the bottomOne click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes
At the bottomAutomatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony
At the bottomManual precise measurement: length, angle, radian, area, arc, any curve

4、 Database module
At the bottomData storage and intelligent query
At the bottomData export: Export statistical results in an Excel spreadsheet
At the bottomData Security: Operator Access Permissions,Data modification permission settings

5、 Instrument specifications and configuration
At the bottomicount 30F/30D1 host
At the bottomColony analysis software
Ø Brand Business LCD Computer