Antibacterial zone measurement and colony counter

WhatThe series is the fourth generation intelligent antibacterial zone measurement and colony counter launched by Xunshu Technology in 2013, designed specifically for large and medium-sized microbial testing institutions. The newly designed enclosed dark box, three color LED structured light, 360 degree surround diffuse lighting, crystal sharp suspended dark field, high fidelity lens, and 10 million pixel camera provide necessary lighting and shadow conditions for high-precision antibacterial zone measurement and colony counting. The entire series is equipped with dual wavelength ultraviolet to meet the needs of disinfection and fluorescent colony excitation.
Czone7DSpecially designed for drug microbiological testing, it integrates level set multi model image segmentation algorithm and Szone multi-mode inhibition zone measurement technology, which can effectively solve common problems in drug testing such as bacterial counting, drug residue interference, microbial limit testing, and antibiotic potency determination.

Fully enclosed lighting
Adopting fully enclosed, wide light strip lighting technology and ergonomic porthole door design, it isolates the interference of ambient light, eliminates the light spot and halo phenomenon formed by stray light refracted in glass culture dishes, and provides * light and shadow conditions for accurate colony counting.

Up and down light source scene lighting
Upper light source: 360 degree flexible mixed light illumination, highlighting the color and texture of the colony, making the wrinkles, depressions, and jagged edges of the colony surface more three-dimensional;
Lower light source: Crystal Sharp suspended dark field illumination can increase the contrast between the colony and the culture medium, clearly outlining the outline of the colony.

Background switching
Suspended dark field is suitable for most culture dishes and can significantly improve the sharpness of images, reflecting tiny deep colonies or impurities. When there are dark colonies in the culture dish, you can switch to a white background to highlight the dark colonies.

Tri color LED light source
Long life, low power consumption, environmentally friendly three color LED mixed light, restores the true color of bacterial colonies, and eliminates the problem of blue bias in white LED lighting imaging.

Free switching of lighting modes
The system adopts a dual light source design: the upper light source uses a multidimensional large array LED mixed light module, which creates a 360 degree surround diffuse soft light through a flexible light guide plate; The bottom light source adopts Crystal Sharp suspended dark field illumination. The upper and lower light sources can be freely switched, and the brightness can be adjusted according to user needs.

Dual wavelength ultraviolet
Built in 254nm ultraviolet lamp can solve the pollution problem caused by the use of colony analyzer * and also meet the needs of ultraviolet mutagenesis.
The dual side 366nm ultraviolet illumination design can excite colony fluorescence, meeting the needs of counting Escherichia coli and observing green fluorescent protein.

One click intelligent mode
Provide 6 one click statistical modules that can accurately segment and count colonies in different culture dishes with just one click, making colony statistics simple and convenient.

Common colony count

Bacterial count
The characteristics of bacterial colonies are extremely rich, such as smooth or wrinkled surfaces, neat or serrated edges, and mutual adhesion, which makes colony statistics extremely difficult. Xunshu's innovative level set fast active contour model can solve the segmentation problem of different types of bacterial images in a targeted manner.

Grid filter membrane
When performing colony counting on filter membranes, traditional image processing methods segment grids rather than colonies. The "Level Set Active Contour Model Based on Morphological Constraints" segmentation algorithm launched by Xunshu Innovation can achieve one click colony counting under grid background.

Spiral petri dish
Designed according to the spiral counting rule of the US FDA, it complies with the entry and exit inspection and quarantine industry standard of the People's Republic of China - "Bacterial Counting Inspection Method for Food and Cosmetics - Spiral Plate Method", and supports the vaccination mode requirements of multiple brands of spiral inoculants, such as SBI in the United States and IUL in Spain.

Microbial limit analysis tool

Applicability check of culture medium
The suitability test of culture medium is to compare the growth status or characteristics of positive bacteria between the test medium and the control medium to evaluate whether the test medium meets the testing requirements. Judgment criteria: The ratio of the average number of colonies on the tested culture medium to the average number of colonies on the control culture medium should be greater than 70%, and the morphology and size of the colonies should be the same as those on the control culture medium. Xunshu provides a simple and efficient statistical tool for microbial limit analysis, suitable for solving the applicability of bacterial, fungal, and yeast counting culture media. With just a simple mouse click, colony statistics and comparative analysis of culture media can be completed, and the results can be exported to EXCEL.

Control bacterial examination - colony morphology
When establishing a microbial limit testing method for drugs, validation of the control bacteria testing method should be conducted to confirm that the method used is suitable for the control bacteria testing of the drug. Xunshu provides a systematic colony morphology description tool for the validation experiment of bacterial inspection methods, avoiding the diversity of description results caused by personal language habits. The software provides a comprehensive library of colony morphology feature description information, and users only need to select with the mouse to complete target colony extraction and feature description, and export the results to EXCEL.

Impurity removal statistics

By switching backgrounds, the contrast between colonies and impurities can be increased, thereby achieving accurate identification of colonies. Like brown traditional Chinese medicine powder, under suspended dark field lighting conditions, the brown powder blends with a black background, highlighting gray white bacterial colonies and avoiding interference from drug residues.

data management

The system adopts an open experimental report printing method, which can export statistical information to EXCEL and edit the report format according to industry needs. To protect the authenticity and originality of experimental data, the system sets three passwords: program startup password, database password, and administrator password.

Antibacterial zone measurement module

ZoneMulti mode measurement technology for antibacterial zone
The software provides three fast antibacterial zone measurement algorithms: automatic detection, quasi circle approximation, and manual detection.

Antibiotic potency determination
Meets the requirements of the Chinese Pharmacopoeia 2010 edition for the two dose method, three dose method, and combined calculation. High definition imaging and high-precision digital measurement ensure the accuracy of potency determination.
The relative error of repetitive self check is less than 0.01%; The relative error of uniformity self-test is less than 0.05%; The accuracy of potency measurement is greater than 99.7%.

Main functions and technical indicators

1、 Lighting system
1. light source
At the bottomVisible light: High brightness tri color LED structured light
At the bottom254nmUV: Used for cavity disinfection and UV mutagenesis
At the bottom366nmUV: Excitation of Escherichia coli, Escherichia coli flora fluorescence, green fluorescent protein
2. Light Path and Lighting Control
At the bottomFully enclosed dark box: eliminating environmental stray light interference
At the bottomUpper light source: Scene style 360 ° flexible shadowless lighting
At the bottomLower light source: Crystal Sharp suspended dark field illumination
At the bottomUp light, down light, dual light, UV, freely switchable, adjustable light intensity

2、 Digital imaging
At the bottomStandard definition industrial fixed focus lens
At the bottom1000Ten thousand pixel professional CMOS camera

3、 Colony analysis module
1. Basic colony counting function
At the bottomType of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet
At the bottomOne click intelligent counting (6 modes)
At the bottomWhole dish colony count: Count the total number of colonies and display them classified by 25 sizes
At the bottomRegion selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics
At the bottomDiameter classification statistics: Set the diameter range and count specific sizes of colonies
At the bottomMouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes
At the bottomColony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies
2. Advanced colony counting function
At the bottomSpiral colony statistics
At the bottomDynamic adjustment statistics
At the bottomDeviation estimation statistics
At the bottomLevel Set Multi Model Algorithm
At the bottomSpecific colony statistics
At the bottomTrans statistics
At the bottomRemoval of miscellaneous bacteria and impurities
At the bottomMulti-color automatic clustering
3. Grid filter membrane and 3M test piece
At the bottomBlack solid line grid, one click statistics
At the bottom3MTotal bacterial count test piece, 3M * test piece: one click statistics
At the bottom3MColiform test strip, 3M Escherichia coli/coliform rapid test strip: one click statistics+manual selection
4. Microbial limit analysis tool
At the bottomApplicability check of culture medium
At the bottomControl bacterial examination - colony morphology
5. Advanced Tools
At the bottomGrid cleaning: eliminate background interference in the filter membrane grid
At the bottomManual counting correction: adding or deleting colonies
At the bottomExclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area
At the bottomBackground text elimination: automatically eliminates marker interference
At the bottomArtificial adhesion segmentation: manually segmenting multiple adhesive colonies
At the bottomAutomatic parameter conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion
At the bottomText and graphic annotations
6. Calibration and Measurement
At the bottomInstrument calibration: The instrument comes with built-in calibration and manual correction calibration
At the bottomOne click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes
At the bottomAutomatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony
At the bottomManual precise measurement: length, angle, radian, area, arc, any curve

4、 Antibacterial zone analysis module
1. ZoneMulti mode measurement technology for antibacterial zone
At the bottomAutomatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones
At the bottomQuasi circular approximation: Based on the contour of the antibacterial zone, circular fitting approximation is suitable for edge rupture and non-standard circular antibacterial zones
At the bottomManual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges
2. Antibiotic potency determination
At the bottomOne dose method potency testing: suitable for the United States Pharmacopeia
At the bottomTwo dose method, three dose method, and combined calculation: suitable for the Chinese Pharmacopoeia 2010 edition
At the bottomRepetitive self check: relative error ≤ 0.01%, repeated measurement accuracy ≤ 0.002mm
At the bottomUniformity self check: relative error ≤ 0.05%
At the bottomMeasurement difference between stations ≤ 0.2%

5、 Database module
At the bottomData storage and intelligent query
Ø NumberExport: The statistical results are exported in an Excel spreadsheet
At the bottomData Security: Operator Access Permissions, Data Modification Permission Settings

6、 Instrument specifications and configuration
At the bottomCharacter 7D1 host
At the bottomColony analysis software, automatic inhibition zone measurement software, antibiotic potency determination software
At the bottomBrand Business LCD Computer