Antibacterial zone measurement and colony counter

WhatThe series is the fourth generation intelligent antibacterial zone measurement and colony counter launched by Xunshu Technology in 2013, designed specifically for large and medium-sized microbial testing institutions. The newly designed enclosed dark box, three color LED structured light, 360 degree surround diffuse lighting, crystal sharp suspended dark field, high fidelity lens, and 10 million pixel camera provide necessary lighting and shadow conditions for high-precision antibacterial zone measurement and colony counting. The entire series is equipped with dual wavelength ultraviolet to meet the needs of disinfection and fluorescent colony excitation.
Character 5FThe configured analysis module is suitable for the needs of microbial detection in food, environment, water quality, antibacterial and mildew prevention.

Tri color LED light source
Long life, low power consumption, environmentally friendly three color LED mixed light, restores the true color of bacterial colonies, and eliminates the problem of blue bias in white LED lighting imaging.

Dual wavelength ultraviolet
Built in 254nm ultraviolet lamp can solve the pollution problem caused by the use of colony analyzer * and also meet the needs of ultraviolet mutagenesis.
The dual side 366nm ultraviolet illumination design can excite colony fluorescence, meeting the needs of counting Escherichia coli and observing green fluorescent protein.

Up and down light source scene lighting
Upper light source: 360 degree flexible mixed light illumination, highlighting the color and texture of the colony, making the wrinkles, depressions, and jagged edges of the colony surface more three-dimensional;
Lower light source: Crystal Sharp suspended dark field illumination can increase the contrast between the colony and the culture medium, clearly outlining the outline of the colony.

Free switching of lighting modes
The system adopts a dual light source design: the upper light source uses a multidimensional large array LED mixed light module, which creates a 360 degree surround diffuse soft light through a flexible light guide plate; The bottom light source adopts Crystal Sharp suspended dark field illumination. The upper and lower light sources can be freely switched, and the brightness can be adjusted according to user needs.

Fully enclosed lighting
Adopting fully enclosed, wide light strip lighting technology and ergonomic porthole door design, it isolates the interference of ambient light, eliminates the light spot and halo phenomenon formed by stray light refracted in glass culture dishes, and provides * light and shadow conditions for accurate colony counting.

Background switching
Suspended dark field is suitable for most culture dishes and can significantly improve the sharpness of images, reflecting tiny deep colonies or impurities. When there are dark colonies in the culture dish, you can switch to a white background to highlight the dark colonies.

Colony count of common petri dishes

Bacterial count
The characteristics of bacterial colonies are extremely rich, such as smooth or wrinkled surfaces, neat or serrated edges, and mutual adhesion, which makes colony statistics extremely difficult. Xunshu's innovative level set fast active contour model can effectively solve the segmentation problem of different types of bacterial images.

Count of mold or actinomycetes
The complex surface texture is a common feature of fungi and actinomycetes, mainly manifested as multiple folds, rough edges, loose or dense hyphae, and different colors in the middle and edges. For this type of colony, Xunshu used the eight chain code contour tracking method and random Hough circle detection method to perform splitting operations on the single level set region, achieving good image segmentation results.

Mold and yeast counts
The food standard requirement for "counting mold and yeast" is to count mold and yeast separately after culturing in Bengal red medium or potato glucose agar medium at 28 ℃. Xunshu "level set multi model search algorithm" can achieve one click statistics of a type of bacteria.

Escherichia coli count
According to the standard for counting coliform bacteria, crystal violet neutral red bile salt agar was used for plate counting of coliform bacteria. The colonies appeared purple red with red bile salt precipitation rings around them. Xunshu's "one click intelligent mode" can complete the counting of coliform bacteria with just one click.

Count of Escherichia coli
According to the standard for counting Escherichia coli, colonies emitting light blue fluorescence on the counting plate are counted under 360 nm to 366 nm wavelength ultraviolet light irradiation in a dark room. The new generation colony analyzer of Xunshu is equipped with a 366nm ultraviolet lamp, which can excite the fluorescence of Escherichia coli for easy observation and counting.

*Inspection
Xunshu addresses the * testing issue on Baird Parker tablets by utilizing optimized statistical algorithms to eliminate interference from transparent circles and achieve accurate colony counting.

Lactic acid bacteria test
The main lactic acid bacteria in the standard are Lactobacillus, Bifidobacterium, and Streptococcus.
The "one click intelligent mode" of Xunshu can also achieve accurate counting of the total number of lactic acid bacteria on MRS agar plates.

Grid filter membrane and 3M test piece
When performing colony counting on 3M test strips or filter membranes, traditional image processing methods segment grids rather than colonies. The "Level Set Active Contour Model Based on Morphological Constraints" segmentation algorithm launched by Xunshu Innovation can achieve one click colony counting under grid background.

Spiral petri dish
Designed according to the spiral counting rule of the US FDA, it complies with the entry and exit inspection and quarantine industry standard of the People's Republic of China - "Bacterial Counting Inspection Method for Food and Cosmetics - Spiral Plate Method", and supports the vaccination mode requirements of multiple brands of spiral inoculants, such as SBI in the United States and IUL in Spain.

mold detection

The standard specifies the testing method for the anti mold performance of plastics, and the mold area is generally estimated through visual observation and measurement with a vernier caliper. In order to accurately determine the anti mold performance of plastic products, Xunshu provides a rapid mold growth level assessment tool - anti mold detection. By automatically extracting mold and sample contours, relevant values can be accurately calculated.

Impurity removal statistics

Impurities often exist in the process of microbial colony counting, such as unfiltered food samples directly injected into culture dishes, and the presence of insoluble substances, bubbles, agar clots, etc. in the culture medium. By utilizing the morphological differences between bacterial colonies and impurities, such as size, color, contour, etc., certain conditions can be set to filter out specific impurities.

data management

The system adopts an open experimental report printing method, which can export statistical information to EXCEL and edit the report format according to industry needs. To protect the authenticity and originality of experimental data, the system sets three passwords: program startup password, database password, and administrator password.

Antibacterial zone measurement module

ZoneMulti mode measurement technology for antibacterial zone
The software provides three fast antibacterial zone measurement algorithms: automatic detection, quasi circle approximation, and manual detection.

Sulbactam sensitive β - lactase test
In order to test whether β - lactase is added to milk, Xunshu provides a fast measurement and intelligent judgment software, which intelligently judges whether β - lactase is positive or negative through pure water verification, parallel sample detection, and average value calculation.

Main functions and technical indicators

1、 Lighting system
1. light source
At the bottomVisible light: High brightness tri color LED structured light
At the bottom254nmUV: Used for cavity disinfection and UV mutagenesis
At the bottom366nmUV: Excitation of Escherichia coli, Escherichia coli flora fluorescence, green fluorescent protein
2. Light Path and Lighting Control
At the bottomFully enclosed dark box: eliminating environmental stray light interference
At the bottomUpper light source: Scene style 360 ° flexible shadowless lighting
At the bottomLower light source: Crystal Sharp suspended dark field illumination
At the bottomUp light, down light, dual light, UV, freely switchable, adjustable light intensity

2、 Digital imaging
At the bottomStandard definition industrial fixed focus lens
At the bottom1000Ten thousand pixel professional CMOS camera

3、 Colony analysis module
1. Basic colony counting function
At the bottomType of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet
At the bottomOne click intelligent counting (6 modes)
At the bottomWhole dish colony count: Count the total number of colonies and display them classified by 25 sizes
At the bottomRegion selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics
At the bottomDiameter classification statistics: Set the diameter range and count specific sizes of colonies
At the bottomMouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes
At the bottomColony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies
2. Advanced colony counting function
At the bottomSpiral colony statistics
At the bottomDynamic adjustment statistics
Ø DeviationDifferential estimation statistics
At the bottomLevel Set Multi Model Algorithm
At the bottomSpecific colony statistics
At the bottomTrans statistics
At the bottomRemoval of miscellaneous bacteria and impurities
At the bottomMulti-color automatic clustering
3. Grid filter membrane and 3M test piece
At the bottomBlack solid line grid, one click statistics
At the bottom3MTotal bacterial count test piece, 3M * test piece: one click statistics
At the bottom3MColiform test strip, 3M Escherichia coli/coliform rapid test strip: one click statistics+manual selection
4. specialized analysis
At the bottomMold proof testing: quantitative analysis of mold proof level
5. Advanced Tools
At the bottomGrid cleaning: eliminate background interference in the filter membrane grid
At the bottomManual counting correction: adding or deleting colonies
At the bottomExclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area
At the bottomBackground text elimination: automatically eliminates marker interference
At the bottomArtificial adhesion segmentation: manually segmenting multiple adhesive colonies
At the bottomAutomatic parameter conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion
At the bottomText and graphic annotations
6. Calibration and Measurement
At the bottomInstrument calibration: The instrument comes with built-in calibration and manual correction calibration
At the bottomOne click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes
At the bottomAutomatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony
At the bottomManual precise measurement: length, angle, radian, area, arc, any curve

4、 Antibacterial zone analysis module
1. ZoneMulti mode measurement technology for antibacterial zone
At the bottomAutomatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones
At the bottomQuasi circular approximation: Based on the contour of the antibacterial zone, circular fitting approximation is suitable for edge rupture and non-standard circular antibacterial zones
At the bottomManual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges
2. Sulbactam sensitive β - lactase test
At the bottomPure water verification: Generate inhibition zones based on (A), (B), and (D), D-C≧3， B-A≦3 ， The judgment system is established
At the bottomAutomatically detect the (A), (B), (C), and (D) inhibition zones of three parallel samples and import the data
At the bottomAutomatically calculate the average value of parallel experiments and intelligently distinguish the positive and negative results.
At the bottomInvalid report automatic warning

5、 Database module
At the bottomData storage and intelligent query
At the bottomData export: Export statistical results in an Excel spreadsheet
At the bottomData Security: Operator Access Permissions, Data Modification Permission Settings

6、 Instrument specifications and configuration
At the bottomCharacter 5F1 host
At the bottomBacterial colony analysis software, automatic inhibition zone measurement software, sulbactam sensitive β - lactam enzyme testing software
At the bottomBrand Business LCD Computer