Microscopic cell analysis, colony counting, screening, and antibacterial zone measurement combined instrument

The MF series multifunctional all-in-one machine is composed of a colony and inhibition zone imaging system, a microscopic imaging system, and image analysis software, which helps to achieve intelligence in routine microbial experiments: microscopic cell observation and analysis, automatic colony counting and screening, inhibition zone measurement, and potency analysis. The practical new MF3 is an upgraded product of the original MF series launched by Xunshu Technology, designed specifically for large testing institutions. Equipped with an Olympus CX31 microscope, it can easily capture sharp, clear, and flat microscopic images for easy microscopic observation and analysis.

Microscopic image analysis

Microscopic image acquisition
The "dynamic and static dual channel parallel observation technology" converts microscopic optical images into digital signals, making it easy to observe the movement of microscopic cells on a liquid crystal screen and capture high-definition static images at any time.

Microscopic measurement function
It can accurately measure the diameter, angle, radian, circumference, area of particles, as well as the length of any manually drawn lines, arcs, curves, etc., and automatically generate measurement results.

Image Processing and Editing
Built in 27 image processing methods, such as adaptive enhancement, color component enhancement, image smoothing, edge sharpening, filtering, edge detection, morphological operations, etc., can significantly improve the microscopic observation effect and meet the special needs of users. Text and graphic embedding tools provide assistance for writing scientific research papers.

Automatic particle statistics
Equipped with a large number of statistical functions, it can quickly count particles and cells, such as regional statistics, diameter classification statistics, color recognition statistics, adhesion segmentation, impurity removal, mouse click statistics, etc.

colony counting

Fully enclosed darkroom shooting
Adopting fully enclosed, wide light strip lighting technology and ergonomic porthole door design, it isolates the interference of ambient light, eliminates the light spot and halo phenomenon formed by the refraction of stray light in glass culture dishes, and provides accurate light and shadow conditions for live bacterial counting. The upper light source provides 360 degree flexible mixed light illumination, which can highlight the color and texture of bacterial colonies; The bottom light source is Crystal Sharp suspended dark field illumination, which can clearly outline the outline of bacterial colonies.

Tri color LED hybrid light source, color temperature adjustment
Scientific research aims to accurately reflect the color of bacterial colonies, while white LED illumination tends to produce blue images. Xunshu uses low-power, environmentally friendly three color LED mixed light, and controls the color temperature range from 3500K to 8500k through the ratio of warm and cool light, capturing the most realistic color of bacterial colonies.

Auxiliary light source - dual wavelength ultraviolet
Built in 254nm ultraviolet lamp can solve the pollution problem caused by the use of colony analyzer * and also meet the needs of ultraviolet mutagenesis. The dual side 366nm ultraviolet illumination design can excite colony fluorescence, meeting the observation of Escherichia coli, green fluorescent protein, etc.

Common colony count
Xunshu is a common colony counting problem for petri dishes, such as bacteria, mold, actinomycetes, mold and yeast, coliform bacteria, *, grid filter membranes, 3M test strips, spiral petri dishes, etc. It provides convenient and fast one click intelligent statistics and advanced statistical tools.

Complex colony counting - mixed bacteria
Some culture dishes may experience mixed growth of bacteria, mold, yeast, and actinomycetes, making colony counting very difficult. The segmentation algorithm suitable for certain types of bacteria may not be suitable for other bacteria. The "trans statistics" module of Xunshu is designed specifically to solve such complex problems, and can achieve accurate segmentation and statistics of colonies in mixed petri dishes with multiple bacteria.

Complex colony counting - colonies are similar to culture medium
Microbial experiments often encounter situations where the color of colonies is very similar to the culture medium, and general algorithms are difficult to achieve accurate identification and segmentation of colonies. The multi processing algorithm of the rapid counting colony analyzer can provide users with more solutions.

Complex colony counting - halo interference
During the process of microbial cultivation, soluble pigments or hemolytic circles often appear around the colony, and the "Multiphase Horizontal Set Activity Profile Model" can effectively solve such problems.

Complex colony counting - chromogenic medium
The sensitivity and specificity of microbial screening and isolation using chromogenic culture medium are significantly better than traditional culture medium. The "RGB Constrained Level Set Active Contour Model" of Xunshu is suitable for the identification and statistics of chromogenic pathogens.

Impurity removal statistics - morphological filtering
Impurities often exist in the process of microbial colony counting, such as unfiltered samples directly injected into culture dishes, and the presence of insoluble substances, bubbles, agar clots, etc. in the culture medium. By utilizing the morphological differences between bacterial colonies and impurities, such as size, color, contour, etc., certain conditions can be set to filter out specific impurities.

Diversity of Impurity Removal Statistics Intelligent Recognition Algorithms
The diversity of intelligent recognition algorithms for rapid counting of bacterial colonies provides rich tools for the removal of impurities and miscellaneous bacteria. Dynamic adjustment statistics can accurately identify actinomycetes without interference from insoluble components in the culture medium.

Impurity Removal Statistics - Exposure Control
Usually, after 48 hours of cultivation (or longer), the colonies are clearly visible. Properly controlling the light intensity and shortening the exposure time can fully display the colonies, while small impurities are filtered out due to insufficient exposure.

Impurity removal statistics - background switching
By switching backgrounds, the contrast between colonies and impurities can be increased, thereby achieving accurate identification of colonies. Like brown traditional Chinese medicine powder, under suspended dark field lighting conditions, the brown powder blends with a black background, highlighting gray white bacterial colonies and avoiding interference from drug residues.

Colony screening

Double circle analysis
Xunshu provides specialized characteristic analysis tools for double circle problems such as antibacterial circle, transparent circle, color changing circle, and growth circle. By accurately measuring the outer circle diameter and colony diameter, the area ratio and diameter ratio of the two are automatically calculated. Automatically sort and locate corresponding colonies based on the size of the ratio, which can be used for screening antibiotics, enzyme preparations, organic acids, etc.

Strain screening - analysis of morphological variation of bacterial cells
There is a certain correlation between the morphological variation of some bacterial strains and the variation in yield. In screening work, efforts should be made to capture and utilize these direct morphological characteristic changes as much as possible to screen out mutant strains. Xunshu is based on the level set active contour model theory, which utilizes the small differences in colony size, contour, color, and other features to accurately identify target colonies.

One click measurement of mold
The traditional fungal research experiments, such as mycelial growth rate, fungal growth amount, mycelial growth inhibition rate, and indoor toxicity determination, use the cross method to measure the colony growth diameter. Due to the spread, looseness, and irregular edge divergence of most fungal colonies, there is a large human error in measurement, resulting in low efficiency. The "one click mold measurement" module of Xunshu can instantly measure the area, circumference, length, and length of large molds by clicking once on the edge of the colony with a "magic wand".

Antibacterial zone measurement

ZoneMulti mode measurement technology
The tube disk diffusion method requires a circular and clear edge of the antibacterial zone, but there may still be situations such as unclear or broken edges in the experiment. The S-zone antibacterial zone multi-mode measurement technology uses three different high-speed algorithms to accurately extract the pixels that make up the edge of the antibacterial zone, thus completing high-precision measurement.

Antibiotic potency determination
Meets the requirements of the Chinese Pharmacopoeia 2010 edition for the two dose method, three dose method, and combined calculation. High definition imaging and high-precision digital measurement ensure the accuracy of potency determination. The relative error of repetitive self check is less than 0.01%; The relative error of uniformity self-test is less than 0.05%; The accuracy of potency measurement is greater than 99.7%.

Sulbactam sensitive β - lactase test
The issue of antibiotic residues has become one of the important factors affecting the safety of dairy products. In order to test whether β - lactase is added to milk, Xunshu provides a fast measurement and intelligent judgment software, which intelligently judges whether β - lactase is positive or negative through pure water verification, parallel sample detection, and average value calculation.

Main functions and technical indicators

1、 Digital imaging of bacterial colonies and inhibition zones
1. Light source
Visible light: High brightness tri color LED structured light
254nm UV: used for cavity disinfection and UV mutagenesis
366nm UV: excitation of Escherichia coli, coliform bacteria fluorescence, green fluorescent protein
2. Light path and lighting control
Fully enclosed dark box: eliminating environmental stray light interference
Upper light source: Scene style 360 ° flexible shadowless lighting
Lower light source: Crystal Sharp suspended dark field illumination
Up light, down light, dual light, UV, freely switch
Color temperature adjustable (3500K-8500K), light intensity adjustable
3. Optoelectronic conversion
High definition industrial fixed focus lens
10 million pixel professional CMOS camera

2、 Colony counting and screening
1.Basic colony counting function
Type of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet, porous plate
One click intelligent counting (6 modes)
Whole dish colony count: Count the total number of colonies and display them classified by 25 sizes
Region selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics
Diameter classification statistics: Set the diameter range and count specific sizes of colonies
Mouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes
Colony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies
2. Advanced colony counting function
Spiral colony statistics
Dynamic adjustment statistics
Deviation estimation statistics
Level Set Multi Model Algorithm
Specific colony statistics
Trans statistics
Statistics of high adhesion bacteria
3. Grid filter membrane and 3M test piece
Black solid line grid, one click statistics
3M total bacterial count test piece, 3M * test piece: one click statistics
3M coliform test strip, 3M Escherichia coli/coliform rapid test strip: one click statistics+manual selection
4. Typical bacterial screening
Removal of miscellaneous bacteria and impurities
Monochrome classification statistics
Multi-color automatic clustering
Multi-color screening
Analysis of Transparent Ring Characteristics
Dual color circle automatic filtering
5. Description of colony characteristics
Digital description of bacterial, yeast, mold, actinomycetes, and colony characteristics
6. Special analysis
Serial statistics
Parallel statistics
7. Advanced Tools
Grid cleaning: eliminate background interference in the filter membrane grid
Manual counting correction: adding or deleting colonies
Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area
Background text elimination: automatically eliminates marker interference
Background stripe removal: automatically eliminates interference from stains on the culture dish
Artificial adhesion segmentation: manually segmenting multiple adhesive colonies
Automatic parameter conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion
Text and graphic annotations
8. Calibration and Measurement
Instrument calibration: The instrument comes with built-in calibration and manual correction calibration
One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes
Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony
Manual precise measurement: length, angle, radian, area, arc, any curve

3、 Measurement and Analysis of Antibacterial Zone
1.ZoneMulti mode measurement technology for antibacterial zone
Automatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones
Quasi circular approximation: Based on the contour of the antibacterial zone, circular fitting approximation is suitable for edge rupture and non-standard circular antibacterial zones
Manual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges
2. Determination of antibiotic potency
One dose method potency testing: suitable for the United States Pharmacopeia
Two dose method, three dose method, and combined calculation: suitable for the Chinese Pharmacopoeia 2010 edition
Repetitive self check: relative error ≤ 0.01%, repeated measurement accuracy ≤ 0.002mm
Uniformity self check: relative error ≤ 0.05%
Measurement difference between stations ≤ 0.2%
3. Shubatan sensitive β - lactase test
Pure water verification: Generate inhibition zones based on (A), (B), and (D), D-C≧3， B-A≦3 ， The judgment system is established
Automatically detect the (A), (B), (C), and (D) inhibition zones of three parallel samples and import the data
Automatically calculate the average value of parallel experiments and intelligently distinguish the positive and negative results.
Invalid report automatic warning

4、 Database and image processing module
1.Image Processing and Editing
Image adjustment, adaptive enhancement, sharpening, filtering, edge detection, morphological operations
2. Database
Data storage and intelligent query
Data export: Export statistical results in an Excel spreadsheet
Data Security: Operator Access Permissions, Data Modification Permission Settings

5、 Microscopic Cell Analysis Module
1.microscopic imaging
Microscope: Olympus CX31 microscope
5 million pixel professional microscope CMOS camera
Resolution: 0.5-1.0 microns
2. Image display and conversion
Image display: Real time dynamic observation, capturing images of any field of view at any time
Image observation: with functions of rotation, enlargement, reduction, mirror conversion, and local observation
Image editing: It has functions such as cutting, copying, pasting, and text input for any area of the image
3. Microscopic image processing
Adaptive enhancement: By performing resolution enhancement processing that matches the features of the original image, the image is made clearer and the edges are more prominent, in order to
Observe and recognize the subtle structure of images.
Image adjustment: arbitrary adjustment of image brightness, contrast, saturation, RGB three colors, conversion of grayscale and negative phase diagrams
Image compensation: Compensate for the distorted parts of the image through various mathematical methods such as linear compensation, logarithmic compensation, Bell compensation, etc,
Make the image clearer.
Image sharpening: By enhancing the high-frequency components of an image, the edges of the image become clearer.
Image smoothing: Through image smoothing processing, the background of the image is made uniform.
Image filtering: Six filtering methods, including Gaussian filtering, low-pass filtering, and median filtering, effectively improve image clarity.
Edge detection: Two detection methods and three operators combined with multiple detection options can more accurately extract image contours.
Morphological processing: Nonlinear mathematical morphological processing such as corrosion, expansion, opening, and closing.
4. Target measurement
Calibration: It has the function of online calibration of the system to achieve precise measurement (default calibration values are built into the system)
Measurement function: online measurement of particle diameter, length, curvature, angle, any curve, area, etc
5. Particle statistics
Automatic statistics: Automatically count particles and display morphological parameters such as area, perimeter, diameter, roundness, etc. of each particle
Regional statistics: You can choose any shape of region such as rectangle, circle, umbrella, etc. for statistics
Diameter classification statistics: Set the diameter range to count particles of specific sizes
Color recognition statistics: Filter specific particles based on chromaticity, brightness, and saturation
Mouse click statistics: Adding or deleting particles with mouse clicks is convenient and fast
Adhesive segmentation processing: According to user needs, particles that adhere to each other can be automatically or manually segmented
Multiple statistical algorithms: using multiple segmentation algorithms, suitable for particle statistics with different backgrounds
Multivariate statistics: comprehensive statistics of multiple microscopic images
Automatic parameter conversion: Based on the statistical area and sample dilution, achieve automatic conversion
6. Drawing and Annotation
Drawing: You can draw lines, rectangles, circles, and any curves as needed for the opened image
Text editing: Text editing of opened images
Annotation: Convenient for annotating lines and angles

6、 Instrument specifications and configuration
newMF3Multi functional all-in-one machine host
OLYMPUS CX31 Trinocular Biological Microscope, Camera Adapter
Microscope electronic eyepiece (5-megapixel CMOS)
Bacterial colony analysis software, automatic inhibition zone measurement software, antibiotic potency determination software, sulbactam sensitive β - lactase testing software
Microscopic analysis software
all-in-one computer