C-zone 8 antibacterial zone measurement and colony counter

Czone 8 is a newly launched intelligent antibacterial zone measurement and colony counter by Xunshu Technology in 2017, equipped with audit tracking. It is designed specifically for large and medium-sized enterprise testing institutions.

Designed according to the requirements for food and drug testing computer information systems, toAnti tampering technology, intelligent reconstruction technology of testing environment testing process, watermark signature technologyAs the core, we have built a multi-level and mutually constrained security management guarantee system, ensuring the security of the system, the integrity and authenticity of data, and meeting the requirements of GMP audit tracking.

Czone8 is a detection type with a fully enclosed dark box, three color LED structured light, 360 degree surround diffuse lighting, crystal sharp suspended dark field, and a 10 megapixel camera paired with a large aperture fixed focus lens, ensuring sharp imaging while meeting software analysis modules for food, medicine, environment, water quality, antibacterial and anti-corrosion purposes. It is suitable for powerful comprehensive detection institutions.

Architecture design to ensure system security and data security

The four tiered system architecture of "system administrator, data administrator, operator, and reviewer" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information.

System administrator: responsible for creating and managing accounts and login passwords for all operators and auditors.

Data administrator: responsible for archive management, sealing, and computer database management of all test data.

Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies

Auditor: Responsible for reviewing the test reports submitted by operators.

Watermark signature, tamper proof, intelligent reconstruction of testing process - achieving effective audit tracking

① Multi user password login, electronic signature, computer-generated operation review date;

② All operational procedures, such as images, dilution ratios, statistical tools, parameters used, test values, correction situations, etc., are automatically recorded in the database by the computer and cannot be modified by the operator;

③ By reproducing the testing environment and process, the reviewer can trace all the operations of the operator.

Fully enclosed lighting

Adopting fully enclosed, wide light strip lighting technology and ergonomic porthole door design, it isolates the interference of ambient light, eliminates the light spot and halo phenomenon formed by stray light refracted in glass culture dishes, and provides * light and shadow conditions for accurate colony counting.

Tri color LED hybrid light source, color temperature adjustment

Long life, low power consumption, environmentally friendly three color LED mixed light, with a color temperature range of 3500K-8500k controlled by the ratio of warm and cool light, captures the most authentic colony color.

Scene based lighting

Upper light source: 360 degree flexible mixed light illumination, highlighting the color and texture of the colony, making the wrinkles, depressions, and jagged edges of the colony surface more three-dimensional;

Lower light source: Crystal Sharp suspended dark field illumination can increase the contrast between the colony and the culture medium, clearly outlining the outline of the colony.

One click intelligent six modes

For the convenience of experimenters to quickly and accurately count colonies, six intelligent modes have been optimized and designed based on common tablet types: flat sensing mode, three-dimensional sensing mode, small colony priority, large colony priority, same color colony priority, and culture medium exclusion mode. Colony statistics can be completed with just one click.

Improve the statistical accuracy of mixed bacterial colonies

In colony counting, it is common for bacteria, molds, and actinomycetes with different shapes and colors to be mixed in the culture dish, especially mold colonies, which have loose texture and significant color changes at the center and edges. Xunshu is based on a multi-layer level set framework model segmentation algorithm, which can effectively solve the statistical accuracy of mixed bacteria.

Colony segmentation under multiple adhesions

The mathematical morphology segmentation method based on topology theory can quickly and accurately segment colonies that are multiple adhered to each other.

One click analysis of 3M microbial test strips

3M ™ Petrifilm ™ The colonies formed on the test piece are small and have varying shades of color. Through a high fidelity lens and a 16 megapixel CMOS camera, the tiny colonies can be highlighted. Professional software module can count with one click: 3M ™ Bacterial count test piece, * test piece, yeast test piece. The coliform test strip needs to be automatically counted and manually identified for bubbles.

Solving Difficult Images - Image Segmentation Algorithm Based on Level Set Active Contour Model

Combining the level set method with the active contour model, the active contour continuously approaches the segmentation target while minimizing the energy functional, until the segmentation is completed when the active contour line stops evolving.

Spiral colony count

The spiral plate counting method conforms to the entry-exit inspection and quarantine industry standard (SN/T 2098-2008) and the US FDA standard, and supports the index mode, slow index mode, uniform mode, proportional mode, lawn mode, etc.

Multi mode measurement technology for Szone inhibition zone

The measurement of the inhibition zone often uses the steel ring double disc method, paper plate method, and agar perforation method. Due to various factors in the experimental process, such as the concentration of antibiotic solution, the quality of the culture medium, the pH value, the age of the test bacteria, and the cultivation time, some of the contours of the inhibition zone formed afterwards are clear, while others have blurred or irregular edges and are accompanied by rupture.

Xunshu provides three algorithms: automatic detection, circle approximation, and three-point circle determination, which can adapt to the rapid measurement of different types of antibacterial zones.

Antibiotic potency determination

The determination of antibiotic potency requires that the edge of the inhibition zone image must be clear. The high-definition fixed focus lens (150LP/mm) configured in zone 8, combined with a 16 megapixel industrial CMOS camera, ensures high-definition digital imaging and lays the foundation for the accuracy of potency determination.

According to the requirements of the 2015 edition of the Chinese Pharmacopoeia for the two dose method, three dose method, and combined calculation, the relative error of repeatability self inspection is less than 0.01%; The relative error of uniformity self-test is less than 0.05%; The accuracy of potency measurement is greater than 99.7%.

Main functions and technical indicators

1、 Lighting system

1. Light source

Visible light: High brightness tri color LED structured light

254nm UV: used for cavity disinfection and UV mutagenesis

2. Light path and lighting control

Fully enclosed dark box: eliminating environmental stray light interference

Upper light source: scene style 360 ° flexible shadowless lighting

Lower light source: Crystal Sharp floating dark field illumination

Up light, down light, dual light, UV, freely switch

Color temperature adjustable (3500K-8500K), light intensity adjustable

2、 Digital imaging

High definition industrial fixed focus lens: focal length 8mm, resolution 150lp/mm

Professional CMOS camera: 1/2.33-inch color CMOS sensor, 16 million pixels C-Mount、USB3.0

3、 Colony analysis module

1. Basic colony counting function

Type of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet

One click intelligent counting (6 modes): flat sensing mode, three-dimensional sensing mode, small colony priority, large colony priority, same color bacteria priority, culture medium removal mode

Whole dish colony count: Count the total number of colonies and display them classified by 25 sizes

Region selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics

Diameter classification statistics: Set the diameter range and count colonies of specific sizes

Mouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes

Colony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies

2. Advanced colony counting function

? Spiral colony statistics: automatically count spiral plates according to FDA standards, and support exponential mode, slow exponential mode, uniform mode, proportional mode, lawn mode, etc. Compatible with SBI in the United States and IUL in Spain. Support the entry and exit inspection and quarantine industry standard SN/T 2098-2008

Dynamic adjustment statistics: The statistical results can be dynamically adjusted and corrected to quickly obtain the statistical effect.

Deviation estimation statistics: suitable for situations with multiple and complex colony colors.

Level set multi model algorithm: search operation, obtain * image segmentation effect, adapt to background changes in the culture medium

Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics

Trans statistics: suitable for extremely complex colony types and uniform culture medium background

Removal of miscellaneous bacteria and impurities: Automatic removal of miscellaneous bacteria and impurities based on differences in morphology, size, and color

Multi color automatic clustering: Select color matching accuracy, classify and statistically analyze the color colonies of the entire dish based on color differences

3. Grid filter membrane and 3M test piece

Black solid line grid with one click statistics

3M total bacterial count test piece, 3M * test piece: one click statistics

3M Escherichia coli test strip, 3M Escherichia coli/Escherichia coli rapid test strip: one click statistics+manual selection

4. Microbial limit analysis tool

Applicability test of culture medium (adapted to the Chinese Pharmacopoeia 2015 edition)

Control bacterial examination - colony morphology

5. Special analysis

Mold proof testing: quantitative analysis of mold proof level

Serial statistics: suitable for complex bacterial colonies with uneven background in the culture medium

Parallel statistics: suitable for porous plate, OPKA, SBA analysis

6. Advanced Tools

Grid Clearing: Eliminating background interference in the filter membrane grid

Manual counting correction: adding or deleting colonies

Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area

Background text elimination: automatically eliminates marker interference

Background stripe removal: automatically eliminates interference from stains on the culture dish

Artificial adhesion segmentation: manually segmenting multiple adhesive colonies

Parameter automatic conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion

Text and graphic annotation: various drawing tools and embedded Chinese and English text

7. Calibration and Measurement

Instrument calibration: The instrument comes with built-in calibration and manual correction calibration

One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes

Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony

Manual precise measurement: length, angle, radian, area, arc, any curve

4、 Data Security and Management

1. The four tiered system architecture of "system, data, operation, and review" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information

System Administrator (Top Level): Responsible for creating and managing accounts and login passwords for all operators and auditors. Ensure account and data isolation between operators and between operators and auditors.

Data Administrator (Deputy Senior): Responsible for archive management of all test data and database management of computers. Seal all approved test reports or backup and export original images and test data to ensure data integrity and security.

Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies.

Reviewer: Responsible for reviewing the test reports submitted by operators. Verify the data input and processing process, but have no authority to modify it; Review and return the suspicious report, and request the operator to retest it; The report that has been approved will be archived, and neither the auditor nor the operator has the right to delete it again to ensure the originality and authenticity of the data.

2. Data storage and export

Mainly based on electronic data, record: sample source, number, dilution, petri dish image, recognition effect, count value, statistical tools used, parameter settings, correction status, to ensure complete record information.

Meet quality audit requirements, and the stored electronic data can be printed and output in PDF or Excel format

3. Watermark signature technology, tamper proof technology, and intelligent reconstruction technology for testing processes to achieve effective audit tracking

4. Anti tampering technology

Adopting multi-user login management, the names of all operators and auditors are automatically recorded in the operation process and test report by the system; All operation dates and review dates are automatically generated by the computer to avoid errors or forgeries.

The entire operation process, including colony images, dish size, sample dilution, statistical tools, parameters used, total bacterial count obtained from testing, self checked and corrected total bacterial count, etc., is automatically recorded in the database by the computer and cannot be modified by the operator, providing all real data for subsequent audits.

5. Watermark signature technology

The test report with "approved" will automatically generate electronic signatures for the operator and auditor's accounts, and an anti-counterfeit "approved" watermark will be printed on the report.

6. Intelligent refactoring technology for testing process

The "reviewer" opens the "waiting for review" test record, and the computer automatically restores the entire process and testing environment of the operator, including: images of the culture dish measured at that time, test results, dish size, sample dilution, statistical tools and parameters used, total bacterial count obtained from the test, correction status

By reproducing the testing environment and testing process, the reviewer can trace all the operations of the operator, verify the accuracy of the test results, and achieve the purpose of audit tracking.

5、 Antibacterial zone analysis module

1. Multi mode measurement technology for Szone inhibition zone

Automatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones

Quasi circular approximation: Based on the contour of the antibacterial zone, circular fitting approximation is suitable for edge rupture and non-standard circular antibacterial zones

Manual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges

2. Determination of antibiotic potency

One dose method potency testing: suitable for the United States Pharmacopeia

Two dose method, three dose method, and combined calculation: suitable for the Chinese Pharmacopoeia 2015 edition

Repetitive self check: relative error ≤ 0.01%, repeated measurement accuracy ≤ 0.002mm

Uniformity self check: relative error ≤ 0.05%

Measurement difference between stations ≤ 0.2%

3. Shubatan sensitive β - lactase test

Pure water verification: Generate inhibition zones based on (A), (B), and (D), D-C≧3， B-A≦3 ， The judgment system is established

Automatically detect the (A), (B), (C), and (D) inhibition zones of three parallel samples and import the data

Automatically calculate the average value of parallel experiments and intelligently distinguish the positive and negative results.

Automatic alert for invalid reports

6、 Database module

Data storage and intelligent querying

Data export: Export statistical results in an Excel spreadsheet

Data Security: Operator Access Permissions, Data Modification Permissions Settings

7、 Instrument specifications and configuration

One main unit of C-zone 8 antibacterial zone measurement and colony counter

Bacterial colony analysis software, automatic inhibition zone measurement software, antibiotic potency determination software, sulbactam sensitive β - lactam enzyme testing software

Brand business desktop computer: dual core CPU/4G memory/1T hard drive/DVD drive/21.5 'high-definition screen, Windows 7 system or above