Heparin immunoaffinity column for detecting lactoferrin in dairy products

Heparin immunoaffinity column for detecting lactoferrin in dairy products

This method is applicable to the determination of lactoferrin content in infant formula milk powder, infant formula Rice noodles, prepared milk powder, fermented milk and other foods in the food safety standard - food nutrition fortifier - lactoferrin (GB1903.17-2016). 1. Good recovery rate and fast speed: Compared to the commonly used heparin affinity columns with agarose matrix on the market, SelectCore Heparin uses a monodisperse polyacrylate matrix, which has a faster drop rate. Under the same loading amount, the drop rate can be 1-2 times faster, which accelerates the pre-treatment time; 2. Easy to use, not afraid of drying: The matrix has high mechanical strength and good chemical stability, ensuring recovery even when the small column is dried and wetted again. The recovery rate of the small column state with added scalar and standard was 95.33% for 1.0 mg/mL that had not dried. After drying, it was re wetted at 94.20% for 1.0 mg/mL

We provide regular lactoferrin heparin immunoaffinity columns that meet domestic standards and have product performance comparable to American brand small columns.

Overview

Heparin affinity column can specifically purify lactoferrin in the sample. It uses columnar agarose gel as the solid carrier, and agarose gel is coupled with heparin to form an immunosorbent, which is packed into the column to make an immunoaffinity column. It can specifically purify lactoferrin in the sample. Heparin affinity columns are widely used for the extraction of dairy samples. This method is fast, easy to operate, and highly accurate, playing a crucial role in improving the quality and safety of food.

principle

Heparin immunoaffinity column can specifically purify lactoferrin in samples. After sample pretreatment, lactoferrin is purified by immunoaffinity column, and lactoferrin is specifically adsorbed on the affinity column. Impurities on the immunoaffinity column are removed by eluent, and the eluent is separated from the lactoferrin affinity column by passing through the separation column. Then, the eluent is injected into HPLC or LC-MS for determination.

Affinity column purification program

Activation of affinity column → loading → rinsing → elution → affinity column regeneration step → reuse of affinity column

Heparin immunoaffinity column for detecting lactoferrin in dairy products