Total aflatoxin ELISA detection kit

Aflatoxin is a metabolite of fungi (mainly Aspergillus flavus and Aspergillus parasiticus), mainly found in grains, nuts, cottonseeds, and some products related to human blood and animal feed. Aflatoxin is mainly composed of AFB1, AFB2, AFG1, and AFG2, and the total amount of aflatoxin generally refers to the total amount of these four hormones. They are Class I carcinogens and have been proven to pose great harm to tissues and organs such as the liver and kidneys of humans and animals. They are a highly potent carcinogen. Therefore, it is necessary to conduct timely detection and monitoring, and the current main detection method is high-performance liquid chromatography. （HPLC）
Total aflatoxin ELISA detection kitIt is a rapid quantitative detection kit for the total amount of Aspergillus flavus based on the principle of immune competition detection. It has the advantages of simplicity, speed, and no need for special equipment, and can be used for laboratory testing as well as on-site measurements in farms, feed mixing workshops, etc.

【 Precautions 】

1. If the room temperature is below 20 ℃ or the reagents and samples have not returned to room temperature (20-25 ℃), it will result in lower OD values for all standards.

If the plate holes dry during the washing process, the standard curve may not be linear and the repeatability may be poor. So after washing and drying the board, the next step should be carried out immediately.

3. Each reagent needs to be shaken well before use.

4. The reaction termination solution is 0.5M sulfuric acid, avoid contact with the skin.

5. Do not use expired reagent kits; Do not mix expired reagent kits; Do not exchange reagents from different batch numbers of reagent kits.

6. Storage conditions:

The reagent kit should be stored at 2-8 ℃ and cannot be frozen. The unused microplates should be vacuum sealed again. Standard substances and colorless colorants are sensitive to light, so they should be stored away from light.

7. Signs of reagent deterioration:

If the color of the color reagent indicates that it has deteriorated, it should be discarded. When the absorbance value of 0 standard (450/630nm) is less than 0.5 (A450nm<0.5), it indicates that the reagent may have deteriorated.

After adding the color developing solution, the general color development time is 15-30 minutes. If the color is light, the reaction time can be extended to 35 minutes (or longer), but not exceeding 40 minutes. On the contrary, reduce the reaction time.

9. Total aflatoxin ELISA detection kitThe reaction temperature is 25 ℃. If the temperature is too high or too low, it will cause changes in the detection absorbance value and sensitivity.