Pierce 34080 SuperSignal West Pico luminescent substrate 500 ML

SuperSignal™ West Pico Chemiluminescent Substrate

Item number:34080

description

Thermo Scientific SuperSignal West Pico substrate is an enhanced chemiluminescence (ECL) HRP substrate that can assist users in achieving low Pick level protein detection during immunoblotting analysis.

SuperSignal West Pico substrate can provide bright signals and low Pick level detection sensitivity for immunoblotting experiments using horseradish peroxidase (HRP) conjugates. This ECL substrate is compatible with various membranes, blocking solutions, and wide range antibody dilutions, meeting users' immunoblotting application needs with excellent performance, versatility, and high cost-effectiveness.
Pierce 34080 SuperSignal West Pico luminescent substrate 500 ML
Characteristics of SuperSignal West Pico substrate:

•ECL- Enhanced chemiluminescence substrate for horseradish peroxidase (HRP)
•Low Pick sensitivity- Detect low Pic level protein bands on nitrocellulose or PVDF membranes
•Long signal durationUnder optimized conditions, the imprinted bands incubated with substrates can continuously output detectable light signals for 6 to 8 hours
•Stable reagent- The working fluid remains stable within 24 hours; The reagent kit can be stably stored at room temperature for up to 1 year
•Price Economy- Optimized the concentration conditions for diluted antibodies:
0.2 to 1.0 μ g/mL primary antibody (diluted 1:1000 to 1:5000 times with 1 μ g/mL storage solution)
10 to 50 ng/mL secondary antibody (diluted 1:20000 to 1:100000 times with 1 μ g/mL storage solution)

Specifications

Content and Storage

Sufficient For: 60 mini-gel blots; 5000 cm2of membrane
• Luminol/Enhancer, 250 mL
• Stable Peroxide Buffer, 250 mL

Store at room temperature.