[NEB] M0276L E. coli Poly (A) polymerase 500 units in stock

E. coliPoly (A) polymerase

Item Number

specification

Price

Beijing inventory

Shanghai inventory

#M0276L

500 units

have

have

#M0276S

100 units

have

have

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feature

■Labeling RNA with cordycepin 5 '- triphosphate or ATP
■Add Poly (A) tail for cloning or affinity purification
■Improve the translation efficiency of RNA transfected into eukaryotic cells

Overview

E. coliPoly (A) polymerase catalyzes the addition of AMP converted from ATP to the 3 'end of RNA, and this reaction does not rely on the presence of a template.

source

reorganizeE. coliStrain carrying Poly (A) polymerase gene cloned from Escherichia coli.

reaction conditions

1X Poly (A) polymerase reaction buffer
[50 mM Tris HCl (pH 7.9 @ 25 ℃), 250 mM NaCl, 10 mM MgCl2], added 1 mM ATP, incubated at 37 ℃.
Thermal deactivation: 65 ℃ for 20 minutes.

Provided with enzymes

10X Poly (A) polymerase reaction buffer
10 mM ATP

Warranty Statement

No DNase, RNase, and phosphatase contamination.

Unit definition

1 unit refers to the amount of enzyme required to catalyze the incorporation of 1 nmol AMP into RNA at 37 ℃ for 10 minutes. Unit activity testing conditions, please

concentration

5,000 units/ml。