Phusion ® Ultra fidelity PCR Master Mix (with HF buffer provided) # 00531L

Phusion ® Ultra fidelity PCR Master Mix (with HF buffer provided)

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specification

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#M0531L

500 reactions (50 μ l reaction system)

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#M0531S

100 reactions (50 μ l reaction system)

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Phusion ® Ultra fidelity PCR Master Mix (with HF buffer provided) # 00531L

Phusion polymerase information

Overview

Due to the need for DNA sequence correction after amplification, high fidelity DNA polymerases are crucial for PCR applications. By
Thermo Scientific for NEB production and quality control ® Phusion ultra fidelity DNA polymerase combines high fidelity and * performance, suitable for all PCR applications. Its * structure includes a similarPyrococcusThe enzyme and an incorporation rate enhancing domain are fused together. This combination enhances the fidelity and speed of the polymerization reaction. The available reagents include single enzyme, premix, and reagent kit. Phusion hot start Flex DNA polymerase is available in both single enzyme and premix formulations. Phusion DNA polymerase can be used for amplification of long fragments and is an ideal choice for cloning experiments.

Other dosage forms

Both Phusion and Phusion hot start Flex DNA polymerases are available in premix formulations, with Phusion premix available in HF or GC buffer.

The Phusion PCR kit contains Phusion polymerase, Phusion HF and GC buffer, dNTPs, MgCl2, DMSO, and DNA standards.

concentration

2,000 units/ml。

Phusion Ultra Fidelity The yield of DNA polymerase amplification fragments is higher and the extension time is shorter.Amplify a segment using different polymerases1.2 kblength C. elegansGenomic fragments. All amplification reactions are repeated according to the manufacturer's conditions（30 A cycle30 Second or 2 Minute extension time) and using microfluidic chip technology（microfluidic LabChip® Analysis and testing.

Phusionwarm start Flex DNAPolymerase has the ability to amplify *.Except for the 1.3 kb C. elegans amplification fragment, all other amplification fragment templates are human Jurkat. The amplification length is shown in the gel diagram above. All amplification reactions were repeated according to the manufacturer's conditions (30 thermal cycles) using microfluidic LabChip technology ® Analysis and testing.