Ochratoxin ELISA detection kit

Ochratoxin A is a secondary metabolite formed by fungi of the Aspergillus and Penicillium genera. It is a potent substance found in the kidneys and liver and is widely present in various foods. Grains and their by-products are the main sources of ochratoxin A. Animal experiments have shown that ingestion of feed contaminated with this substance can lead to acute or chronic symptoms. It is important to strengthen the detection of ochratoxin A to prevent food and feed contaminated with it from directly or indirectly entering the human food chain.

Ochratoxin ELISA detection kitUsing competitive ELISA, pre coat the microplate with ochratoxin A antigen, add sample/ochratoxin A standard solution and horseradish peroxidase labeled ochratoxin A antibody. The ochratoxin A in the sample or standard solution competes with the ochratoxin A antigen pre coated on the microplate to bind to the horseradish peroxidase labeled ochratoxin A antibody. Unbound enzyme-linked antibodies are removed during washing, followed by the addition of colorimetric solution and the measurement of absorbance values. The absorbance value of the sample is negatively correlated with the content of residual ochratoxin A antigen. By comparing with the standard curve, the content of residual ochratoxin A can be determined.

【 Precautions 】

1. If the room temperature is below 20 ℃ or the reagents and samples have not returned to room temperature (20-25 ℃), it will result in lower OD values for all standards.

If the plate holes dry during the washing process, the standard curve may not be linear and the repeatability may be poor. So after washing and drying the board, the next step should be carried out immediately.

3. Each reagent needs to be shaken well before use.

4. The reaction termination solution is 0.5M sulfuric acid, avoid contact with the skin.

5. Do not use expired reagent kits; Do not mix expired reagent kits; Do not exchange reagents from different batch numbers of reagent kits.

6. Storage conditions:

The reagent kit should be stored at 2-8 ℃ and cannot be frozen. The unused microplates should be vacuum sealed again. Standard substances and colorless colorants are sensitive to light, so they should be stored away from light.

7. Signs of reagent deterioration:

If the color of the color reagent indicates that it has deteriorated, it should be discarded. When the absorbance value of 0 standard (450/630nm) is less than 0.5 (A450nm<0.5), it indicates that the reagent may have deteriorated.

After adding the color developing solution, the general color development time is 15-30 minutes. If the color is light, the reaction time can be extended to 35 minutes (or longer), but not exceeding 40 minutes. On the contrary, reduce the reaction time.

9.Ochratoxin ELISA detection kitThe reaction temperature is 25 ℃. If the temperature is too high or too low, it will cause changes in the detection absorbance value and sensitivity.