Neuroshellfish ELISA detection kit

Neurotoxic shellfish poisoning (NSP) mainly refers to a type of toxin isolated from a red tide organism, Ptychodiscusbreve. The physicochemical and pharmacological properties of PbTX are similar to those of tigecycline (CTX). PbTx can cause significant mortality in fish, mollusks, crustaceans, sponges, echinoderms, and benthic algae. After being ingested by shellfish, some short naked algae accumulate their hormones in their bodies and are transmitted to humans through the food chain, causing food contamination. The main symptoms include enlarged pupils, unpredictable body temperature, nausea, vomiting, diarrhea, and abnormal movement, but no numbness. Therefore, in order to distinguish it from DSP that causes paralysis, it is called neurogenic shellfish extract. The duration of symptoms in NSP is relatively short, generally ranging from 10 minutes to 20 hours.

Neuroshellfish ELISA kitBased on the competitive ELISA enzyme-linked immunosorbent assay method, antibodies against neuroshellfish protein are encapsulated in enzyme labeled wells. The sample and neuroshellfish protein enzyme label are added to the micropores. If there is residual neuroshellfish protein in the sample, it will compete with the shellfish protein enzyme label for antibody coating on the enzyme-linked immunosorbent assay plate. After adding TMB substrate, the color changes, and the content of neurotoxin in the sample is determined by measuring the reading with an enzyme-linked immunosorbent assay (ELISA) reader. The depth of color is inversely proportional to the residual amount of pigment.

Precautions

1. The standard contains neurotoxin shellfish extract, please use with caution.

2. Do not use expired reagent kits.

3. Do not mix different batches of reagent kits, as antibodies and microplates have box to batch specificity.

4. Try to maintain a room temperature of (25 ± 2.5) ° C and avoid operating at ventilation openings to prevent low temperatures, overheating, and/or evaporation. Similarly, do not conduct experiments under direct sunlight to prevent overheating and evaporation. During the incubation period, if the temperature of the workbench is too low, several tissues or other materials should be laid on top.

5. Water quality is important, ensure the use of distilled or deionized water.

6. When adding samples or reagents to an empty microplate, place the suction nozzle close to the bottom of the microplate and maintain contact.

7. The more standardized the calculation of incubation time, the better. To maintain the consistency of adding standards, add standards first and then samples.

8. Add standard samples from low to high concentrations to reduce the risk of affecting the quality of the standard curve.

9. Place the microplate in a sealed bag with desiccant and store it in the refrigerator.