Mouse gastric fibroblast/immunofluorescence identification

smallMouse gastric fibroblasts/Immunofluorescence identificationdetailed

The stomach wall is generally composed of three layers of tissue, with the inner layer being the mucosal layer, the outer layer being the serosal layer, and the middle layer being the muscular layer composed of smooth muscles. Fibroblasts are common cells in connective tissue that differentiate from mesenchymal cells during embryonic development. One of the main functions of fibroblasts is to synthesize collagen and other extracellular matrix, which play an important role in the process of tissue and organ fibrosis.

Cell characteristics

1) The organization is derived from normal gastric tissue of experimental animals.
2) Cell identification: Fibronectin or Vimentin immunofluorescence staining is positive.
3) After identification, the purity of the cells was found to be higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
5) Cell growth mode: spindle shaped cells, irregular cells, adherent culture.

Mouse gastric fibroblasts/Immunofluorescence identificationTransportation and storage of products

Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

Product Usage

1) This product can only be used for scientific research purposes

2) This product has not been approved for direct use in live animals and humans

3) This product has not passed the review for in vivo diagnosis

The main products and technical services of Saibaikang (Shanghai) Biotechnology Co., Ltd. are:

1、 Primary Cell Services
Multiple primary cell resources of various mammalian models;
Multiple human derived normal and tumor primary cell resources;
Reagents related to primary cell isolation and culture kit、 Culture medium additives, etc;
Primary cell related technical services and overall experimental outsourcing services;

2、 Cell line/lineage services
Cell line/strain culture, proliferation, cryopreservation, and related instructions;
Technical guidance for cell line culture process;
Cell line culture medium, additives, serum and related growth factors, reagents, etc;

3、 IPS cells
Basic culture of iPS cells (with or without trophoblast layer);
IPS cell proliferation and cryopreservation;
IPS Basic Training Technology Internship;
Pre market evaluation of new drugs and experimental instruments;

4、 Technical outsourcing services: various cell biology experiments, molecular biology experiments, microscope photography services, etc

5、 Other: Customized services according to customer needs, etc