Immunofluorescence identification of rabbit gallbladder epithelial cells

Rabbit gallbladder epithelial cells Immunofluorescence identificationdetailed

The gallbladder is a pear shaped pouch located behind the liver below the right rib, which has the function of concentrating and storing bile. The gallbladder wall is composed of three layers: mucosa, muscle layer, and outer membrane.
The inner surface of the gallbladder is covered with mucosa and has developed folds. When the gallbladder contracts and empties, the folds are tall and branching; When the gallbladder is full, the folds decrease and become shorter. The mucosal epithelium is a single-layer columnar structure with a small number of dispersed goblet cells. Gallbladder mucosal cells have typical characteristics of absorptive cells, with strong absorption and concentration functions. Epithelial cells absorb water and inorganic salts from bile, which are transported through the cytoplasmic membrane on the cell side into the interstitial spaces of the skin cells. The absorbed water and inorganic salts enter the blood vessels and lymphatic vessels in the lamina propria through the basement membrane. At the same time, the gallbladder mucosa also has a secretion function, secreting mucus.
Cell characteristics
1) The cells are derived from normal gallbladder tissue of experimental animals.
2) Cell identification: Cytokeratin 19 (CK-19) immunofluorescence staining was positive.
3) After identification, the purity of the cells was found to be higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Rabbit gallbladder epithelial cells Immunofluorescence identificationTransportation and storage of products

Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

Product Usage

1) This product can only be used for scientific research purposes

2) This product has not been approved for direct use in live animals and humans

3) This product has not passed the review for in vivo diagnosis

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