Mouse femoral artery smooth muscle cells/immunofluorescence identification

Smooth muscle cells of mouse femoral artery/Immunofluorescence identificationdetailed

The femoral artery is the main trunk of the lower limb arteries, which continues from the external iliac artery. Insert a triangle deep into the midpoint of the inguinal ligament. In the femoral triangle, the femoral artery is first located on the outer side of the femoral vein, gradually crossing from the outer side to the front of the femoral vein, descending into the adductor tube, and then passing through the adductor tendon hiatus to the popliteal fossa, renamed as the popliteal artery. The femoral artery is located superficially at the midpoint of the groin and can be palpated for pulsation. It is the site for emergency compression hemostasis and puncture in clinical practice.

A major factor in the occurrence of arterial disease is the transformation of vascular smooth muscle cells into phenotypes with reproductive ability. Recent studies have shown that smooth muscle cells can express calcium ion channels, ICAM-1, and VCAM-1. The expression of ICAM-1 and VCAM-1 may be the cause of vascular wall inflammation and further contribute to vascular diseases. Therefore, in vitro culture and research of arterial smooth muscle cells can be used to discover and determine targeted therapies for new vascular diseases.

Cell characteristics

1) The organization is derived from normal femoral artery tissue of experimental animals.

2) Cell identification: Smooth muscle actin (α - SMA) immunofluorescence staining was positive.

3) After identification, the purity of the cells was found to be higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: spindle shaped cells, irregular cells, adherent culture.

Smooth muscle cells of mouse femoral artery/Immunofluorescence identificationTransportation and storage of products

Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

Product Usage

1) This product can only be used for scientific research purposes

2) This product has not been approved for direct use in live animals and humans

3) This product has not passed the review for in vivo diagnosis

The main products and technical services of Saibaikang (Shanghai) Biotechnology Co., Ltd. are:

1、 Primary Cell Services
Multiple primary cell resources of various mammalian models;
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Reagents related to primary cell isolation and culture kit、 Culture medium additives, etc;
Primary cell related technical services and overall experimental outsourcing services;

2、 Cell line/lineage services
Cell line/strain culture, proliferation, cryopreservation, and related instructions;
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IPS cell proliferation and cryopreservation;
IPS Basic Training Technology Internship;
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4、 Technical outsourcing services: various cell biology experiments, molecular biology experiments, microscope photography services, etc

5、 Other: Customized services according to customer needs, etc