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Qingdao Shuide Technology Co., Ltd

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info@watertools.cn
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13808986092
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9th Floor, Haidu Business Center, No. 345-2 Zhongcheng Road, Chengyang District, Qingdao City

Liquid automatic injection water sampler

NegotiableUpdate on 01/22

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Nature of the Manufacturer: Producers

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Overview

The German company HYDRO-BBIOS spent three years designing a new intelligent water sampler - AFIS. This water sampler can quickly and evenly inject a certain amount of fixative into the sampling bottle while sampling, which can instantly fix the mRNA of microorganisms in the sample. These fixed mRNAs can be reverse transcribed into DNA in the laboratory, which can then be amplified by PCR to study the selective expression of microorganisms in specific environments. German HYDRO-BBIOS company AFIS liquid automatic injection water sampler

Product Details

German HYDRO-BIOS company AFISLiquid automatic injection water sampler

Automatic water collector based on mRNA underwater in-situ fixation technology

Automatic Fluid Injection Sampler AFIS

German HYDRO-BIOS company AFISLiquid automatic injection water samplerEquipment features:

High pressure injection enables rapid fixation of samples within seconds
Optimal injection system achieves uniform distribution of injected liquid
Users can choose the liquid to be injected
The volume of injected liquid can be edited
The schedule of the sampling process can be edited
Can be used for common multi-channel water sample collection systems

AFIS inventor Dr. Guenter Jost:

Dr. Jost obtained his doctoral degree from Rostock University in 1983 and has served as a German scientist for the Sino German cooperation project "Marine Microbial Ecological Function" (1996-2000), a visiting professor at Cato-lice del Norte Coquimbo University in Chile (1995), Xiamen University Environmental Science Center (1998), and School of Life Sciences (2002). Currently undertaking research on "The Role of Bacteria in the Marine Microfood Web", "Bacterial Activity in Mn and N Circulation at the Aerobic and Anaerobic Interfaces of Water", "Marine Inorganic Nitrifying Bacteria", "Interactions between Water Bacteria and Bacteriophages" in TRUMP, JGOFS, GOBEST, DFG projects. Currently serving as a German scientist in the Sino German cooperation project "Research on the Coexistence System of Indigenous Bacteriophages and Their Host Bacteria in Different Water Environments". Published multiple papers in journals such as ISME, Environment Microbiol, and Appl Environment Microbiol.

Equipment Introduction:

Microorganisms are catalysts that drive almost all biogeochemical cycles. Therefore, understanding the abundance, diversity, and activities of microorganisms in the surrounding environment is not only a fundamental field of research for microbiologists, but also very important for understanding the Earth's basic cycles. The culture free method is used to infer the metabolic function of prokaryotic cells for analyzing the transcriptome.
The major challenge in the metabolism of marine microorganisms is the poor stability of mRNA, which is easily degraded in natural environments. Therefore, it is impossible to objectively study the expression of microbial DNA in specific environments (such as water depth, temperature, salinity, dissolved oxygen, etc.) in laboratory environments.

Spray time, speed, number of nozzles

After repeated testing, we ensure excellent performance

Fixed injection effect

Replace the cap with a spherical valve to seal the sampling bucket and prevent the pressure difference caused by the sealing moment from affecting the activity of bacteria and microorganisms inside the bucket.

Built in reagent bag for easy replacement,

Can be replaced according to different needs

Fixed agent.

Application areas:

AFIS, with its mRNA underwater in situ fixation technology, can serve as a supporting device for bacterial research, biological genetic information transmission, and expression matching. It is a groundbreaking technological breakthrough in marine bacterial and microbial research.
AFIS can be used independently to collect water samples on-site and fix the mRNA of bacteria, microorganisms, etc. in them.
It can be fixed on other large equipment, such as CTD water collectors, ROVs, Landers, etc., to collect water samples simultaneously during other investigation projects.

AFIS is installed on the CTD water sampler

AFIS is mounted on ROV

AFIS is equipped on other devices such as Lander

Perfect compatibility with CTD water sampler:

An important application of AFIS is in conjunction with CTD water collectors. AFIS is perfectly compatible with CTD water collectors widely used by domestic users. In terms of sampling trigger mechanism, hardware installation, etc., CTD water collectors from German company HYDRO-BIOS and American company Sea Bird have successfully achieved perfect compatibility with AFIS and passed testing.

When used in conjunction with the CTD water sampler from the German company HYDRO-BBIOS, simply remove the original card cover water sampler and replace it with AFIS. When used in conjunction with the CTD water sampler from Sea Bird in the United States, the original 2 cap type water sampler bottle positions can be replaced with 1 AFIS.

Not only did it improve the utilization rate of the CTD water collector host, but it also saved valuable research funds.

Technical parameters:

Size: 20 x 20 x 65 cm

Weight in air: 20 kg

Large operating water depth: standard 3000m, optional 6000m

Material: Industrial ceramics, PVDF，PTFE，POM， Titanium, AISI 316 stainless steel, FKM，FFKM

Power supply: LiFePO4 battery, 2500mAh

Supporting protocols: up to 100 operational protocols

Sampling method: Mechanical start, deep start, time start or motion dependent start (programmable) of CTD water sampler

Sampler volume: 2000ml

Injected liquid volume: 250ml large (programmable)

Injection pressure: 700 to 50kPa

The achievements obtained by utilizing AFIS:

Thaumarchaeal distribution and amoA gene expression

The picture shows a chemical profile of the center of the Baltic Sea. The red dots mark the locations of sampling stations 284 (Landsort Deep, 58135.00N, 18114.060E) and 271 (Gotland Deep, 57119.890N, 20110.280E), where the data are various chemical parameters recorded during the sampling period in August 2008 and September 2009. The dashed lines indicate sampling depths of 118 meters (Gotland Deep 2008), 89 meters (Landsort Deep 2008), and 72 meters (Landsort Deep 2009).