FluorCam Closed Chlorophyll Fluorescence Imaging System

PSI scientists Professor Nedbal and Dr. Trtilek combined PAM chlorophyll fluorescence technology with CCD technology and successfully developed and produced the FluorCam chlorophyll fluorescence imaging system worldwide in 1996 (Heck et al., 1999); Nedbal et al., 2000; Govindjee and Nedbal, 2000）。 FluorCam chlorophyll fluorescence imaging technology became an important breakthrough in chlorophyll fluorescence technology in the 1990s, allowing scientists to enter the two-dimensional and microscopic worlds of photosynthesis and chlorophyll fluorescence research. At present, PSI Company has become the professional manufacturer of chlorophyll fluorescence imaging with the highest number of applications, types, and published papers.

The above figure shows the FluorCam chlorophyll fluorescence imaging technology designed by Nedbal et al. in the 1990s (Photosynthesis Research, 66:3-12, 2000). The above figure shows the lemon color image and chlorophyll fluorescence imaging image (Photosynthetica, 38:571-579, 2000)

The FluorCam closed chlorophyll fluorescence imaging system is a highly integrated, innovative, user-friendly, and widely used chlorophyll fluorescence technology device. The high-sensitivity CCD lens, four fixed LED light source boards, and control system are integrated into a dark adaptation operating box. Plant samples are placed on a partition in the dark adaptation operating box, which can be adjusted in height by 7 levels; The light source is powered by a high stability power supply unit, and four high-energy and high stability LED light source boards uniformly illuminate the plant sample, with an imaging area of up to 13 × 13 cm. The control system is connected to a computer through USB and controlled and analyzed data through FluorCam software program. Suitable for plant tissues such as leaves and fruits, whole plants or multiple cultivated plants, lower plants such as mosses and lichens, algae, etc. It is widely used in plant research including algae photosynthetic physiology and ecology, plant stress physiology and susceptibility, stomatal function, plant environment such as soil heavy metal pollution response and biological detection, plant resistance detection and screening, crop breeding, phenotyping, etc.

Main functional features:
The system is integrated into the dark adaptation operation box, which is easy to operate and mobile. It can be used for dark adaptation imaging measurement and analysis both in the laboratory and outdoors
High sensitivity CCD lens with a time resolution of up to 50 frames per second, capable of quickly capturing chlorophyll fluorescence transients and imaging an area of 13x13cm
• It is a chlorophyll fluorescence technology device in the world that can perform OJIP rapid fluorescence dynamic imaging analysis. It can obtain the OJIP rapid chlorophyll fluorescence dynamic curve and 26 parameters including Mo (initial slope of OJIP curve), OJIP fixed area, Sm (measurement of energy required to close all light reaction centers), QY, PI (Performance Index), etc
• It is a chlorophyll fluorescence technology device in the world that can perform QA re oxidation kinetics imaging analysis. It can operate single cycle saturated flash (STF) chlorophyll fluorescence induction dynamics, and the light intensity can reach 120000 µ mol (photons)/m ². s within 100 µ s
A functional and editable chlorophyll fluorescence experimental program (Protocols), including snapshot mode, Fv/Fm, Kautsky induction effect, 2 chlorophyll fluorescence quenching analysis (NPQ) protocols (2 customized light supply schemes), LC light response curve, PAR absorption and NDVI imaging analysis, QA re oxidation kinetics analysis, OJIP rapid fluorescence kinetics analysis, and GFP green fluorescent protein imaging, etc
• It can perform automatic repeated imaging measurement analysis, preset an experimental program (protocols), measurement times, and intervals. The system will automatically cycle the imaging measurement and automatically store the data in the computer according to the time date (with timestamp); Two experimental protocols can also be pre-set; For example, making the system automatically run Fv/Fm during the day and NPQ analysis at night
Equipped with a dual color chemical light excitation source, standard configuration includes red and white, and optional dual band photochemical light such as red and blue. Dual color chemical light can be used in different proportions to experiment with the photosynthetic benefits of crops/plants under different light qualities

The left figure A shows the Fv/Fm of cucumber leaves under 100% red light source conditions, while the left figure B shows the Fv/Fm of cucumber leaves under 30% blue light source conditions; The upper right figure shows the relationship between photosynthesis intensity and light intensity (different proportions of blue light), while the lower right figure shows the relationship between stomatal conductance and light intensity (different proportions of blue light)

Optional TetraCam color imaging module with an imaging area of 20 × 25cm, used for leaf or plant morphology imaging analysis and chlorophyll fluorescence imaging comparative analysis