Centrifuge is a common separation instrument in laboratories, widely used in biomedical, petrochemical, agricultural, food hygiene and other fields.

If you can understand the centrifugal principle of a centrifuge and the determining factors that affect the centrifugal effect, it will help you in choosing a centrifuge and conducting daily centrifugal experiments.

1.

Working principle of centrifuge

The centrifugal machine generates a strong centrifugal force by rotating the rotor around the axis at high speed. Based on the difference in settling velocity of different substances in the centrifugal field, it separates substances with different properties in the sample to meet the analysis and separation requirements of researchers for experimental samples.

2.

Factors affecting centrifugal effect

The separation efficiency of a centrifuge is related to many factors, such as centrifuge performance, centrifugation method, centrifugation medium, and density gradient;

In practical operation, the following conditions are also crucial.

Today, Xiao Ao will introduce something to you:

The influence of temperature and pH of centrifugal media

For biological samples,To prevent aggregation, denaturation, and inactivation of the substance to be separatedIn addition to paying attention to the selection of centrifugal mediaIt is necessary to control the centrifugation conditions such as temperature and pH value of the medium solution.

The centrifugation temperature is generally controlled at around 4 ℃,For some biological samples with good thermal stability, the centrifugation process can be carried out at room temperature.

However, due to overspeed or high-speed centrifugation, the high-speed rotation of the rotor will generate heat, causing an increase in temperature and resulting in sample loss. Therefore, a refrigeration system must be used to stabilize the temperature within a certain range.

The pH value of the centrifugal medium solution should be within the pH range where the sample can stably exist, and buffer solution can be used if necessary.

In addition, excessive acidity or alkalinity of the solution may cause corrosion of the rotor and other components of the centrifuge. It is advisable to avoid direct contact with the components and clean them promptly after contact.

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