Why does the same color scheme and experimental steps result in a 'buyer show' and a 'seller show'.

Let's take a look at his experimental records,

However, when performing intracellular staining, dead cells have a greater impact on the experimental results. However, ordinary PI or 7AAD may result in all cells being positive due to the need for cell fixation and membrane rupture. At this point, we need to use a universal dead or alive cell dye, which can effectively distinguish between dead and alive cells in both intracellular and membrane staining.

Amine-reactive dyes for dead cell discrimination in fixed samples. Curr Protoc Cytom. 2010 Jul; Chapter 9:Unit 9.34. doi: 10.1002/0471142956.cy0934s53.

Comparison with traditional dead or alive cell dyes.

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