We often encounter situations where the dyed markers come from the same literature, but the experimental results turn out to be "buyer show and seller show".

What is the reason for this phenomenon? And why should we optimize our dyeing scheme? Let's first look at specific examples.

Scheme 1 and Scheme 2 both use the same marker * and the same type of fluorescein, but with different combinations. So let's take a look at the same sample and see if the results of each cell subpopulation are the same?

Scheme 1 and Scheme 2 have similar percentages of CD4+CD25+(compared to A and C), but there is a significant difference in the percentage of CD8+CD69+(compared to C and D).

The reason for this is actually the spreading phenomenon that we usually talk about.

Nguyen, Richard, et al. 'Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. ' Cytometry Part A 83.3 (2013): 306-315.

If we want to optimize the resolution reduction caused by the spreading phenomenon in the flow dyeing scheme, we can use the SWOFF method. By following the following process, it is possible to quickly identify which channel's color scheme is unreasonable, resulting in a decrease in resolution.

The photoelectric conversion efficiency of components will directly affect the overall resolution of the instrument on different detection platforms.

Not only that, the advancement of technology has also brought about a smaller spreading phenomenon, which makes color matching easier and results more accurate.

Do you want to know how to check your staining plan? Using the SWOFF method,We can help you discover and optimize, making detection more accurate.Click belowRead the full textThe detailed steps to obtain SWOFF.