Tuner (DE3) Escherichia coli host bacterium

NegotiableUpdate on 01/21

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Overview

Tuner (DE3) Escherichia coli host strain: This strain is derived from BL21 (DE3) strain and was obtained by mutating the lacZY gene (galactosidase gene) on the genome of BL21 (DE3) strain. This mutation allows IPTG from the external environment to enter each E. coli cell in the culture medium at a uniform rate, resulting in a more stringent and uniform concentration dependence of cells on IPTG.

Product Details

Tuner (DE3) Escherichia coli host bacterium

Genotype: F-ompT hsdSB(rB- mB- )gal dcm lacY1(DE3)

This strain is derived from the BL21 (DE3) strain and was obtained by mutating the lacZY gene (galactosidase gene) on the genome of the BL21 (DE3) strain. This mutation allows IPTG from the external environment to enter each E. coli cell in the culture medium at a uniform rate, resulting in a more stringent and uniform concentration dependence of cells on IPTG.

Like its parent strain, this strain integrates a DE3 region on its chromosome, encoding T7 bacteriophage RNA polymerase. When used in conjunction with a vector containing the T7 promoter, the expression level of exogenous proteins on the vector can be adjusted from low to ultra-high levels by precisely adjusting the concentration of IPTG. Especially at low levels of expression, protein solubility may be better.

Cellregen can provideTuner (DE3) Escherichia coli host bacteriumGlycerol bacteria or puncture bacteria

Note:

1. Glycerol bacteria: (1) Transportation method: transportation below 0 ℃ (2) Storage method: -80 ℃ frozen storage, regular (1 year) rejuvenation

2. Puncture bacteria: (1) Transportation method: 2-8 ℃ transportation(2) Storage method: stored at 4 ℃ for 1 week

Product Catalog

Item Number	Product Name	Specifications	Catalog price
PB1311	Tuner (DE3) glycerol bacteria	0.5 mL	850
PB1312	Tuner (DE3) puncture bacteria	branch	800

List of Escherichia coli host bacteria provided by Serruicheng

DH5 α glycerol bacteria/puncture bacteria

*0 glycerol bacteria/puncture bacteria

GM3819 glycerol bacteria/puncture bacteria

TG1 glycerol bacteria/puncture bacteria

HB2151 glycerol bacteria/puncture bacteria

XL Blue glycerol bacteria/puncture bacteria

ER2738 glycerol bacteria/puncture bacteria

BL21(DE3)Glycerol bacteria/puncture bacteria

BL21(DE3)Star glycerol bacteria/puncture bacteria

BLR(DE3)Glycerol bacteria/puncture bacteria

BLR (DE3) pListGlycerol bacteria/puncture bacteria

BL21trxB (DE3)Glycerol bacteria/puncture bacteria

BL21trxB (DE3) pLysSGlycerol bacteria/puncture bacteria

Origami B (DE3)Glycerol bacteria/puncture bacteria

Origami B (DE3)PLysS glycerol bacteria/puncture bacteria

Rosetta(DE3)Glycerol bacteria/puncture bacteria

Rosetta2 (DE3)Glycerol bacteria/puncture bacteria

BSR(DE3)Glycerol bacteria/puncture bacteria

M15 glycerol bacteria/puncture bacteria

Tuner (DE3) glycerol bacteria/puncture bacteria

Rosetta gami B (DE3) glycerol bacteria/puncture bacteria

C43 (DE3) glycerol bacteria/puncture bacteria