This method is a quantitative detection method for ruminant animal derived components (sheep, goats, cows) in animal derived (pig) raw materials using QPCR. The detection limit of this detection method is 0.1 ppm (mass fraction).
Using TaqMan real-time fluorescence PCR technology, trace DNA detection is achieved based on the detection of multi copy genes in ruminants and pigs, which is suitable for adulteration detection of crude animal derived raw materials. Using our company's magnetic bead assay kit to extract DNA, the extractedDNAReal time fluorescence for templatesPCRAmplification, using real-time quantitative detection technology, utilizes single PCR method to prepare the reagents in a pre mixed form to form real-time fluorescencePCRDNA detection kit for pig and ruminant sources. One of the tubes is used to detect the content of pig genes in the sample(FAMThe other tube is used to detect the gene content of ruminant animals in the sample(FAMFluorescence).
□96 Tests/kit
This reagent kit adopts the mode of pre packaging 20ul of reaction solution in the reaction system. Users only need to add 5ul of DNA sample to each well for machine testing, which greatly improves the convenience of use and reduces the probability of cross contamination. Users need to cut out several PCR tubes and melt them at room temperature to avoid repeated freezing and thawing of the entire kit, which may affect the detection efficiency.