Quickly obtaining results makes online detection possible. The sensitivity of fluorescent quantitative PCR method for detecting over 300 types of mycoplasma is essential for biopharmaceuticals and cell therapy. The specificity of mycoplasma detection is designed based on powerful bioinformatics. Positive and negative results are determined by amplification and melting curves, and can be obtained within one working day from sample processing to results, enabling online detection.
Mycoplasma is a common contamination encountered in biopharmaceuticals and cell therapy. Due to its small size, 0.2um filtration cannot eliminate Mycoplasma. The traditional cultivation method has a long cycle and is not suitable for rapid release, making online detection impossible. Conventional PCR methods can only cover a limited number of mycoplasma species, and there is a high possibility of missed detection for trace amounts of mycoplasma. The Taqman probe method also has limited limitations in detecting mycoplasma species. This kit adopts a dye based design and covers over 300 common types of mycoplasma known in the market, achieving as many mycoplasma detections as possible. This kit determines positive and negative results based on the detection results of amplification and melting curves, and can be obtained within one working day from sample processing to results.