Rosetta (DE3) Escherichia coli host bacterium

NegotiableUpdate on 01/21

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Overview

Rosetta (DE3) E. coli host strain: This strain is derived from BL21 (DE3) and can significantly enhance the expression of eukaryotic proteins containing rare codons in E. coli.

Product Details

Rosetta (DE3) Escherichia coli host bacterium

Genotype: F- ompT hsdSB(rB-mB-)dcm gal(DE3) RARE(argU, argW, ileX, glyT, leuW, proL)

This strain is derived from BL21 (DE3) and can significantly enhance the expression of eukaryotic proteins containing rare codons in Escherichia coli.

This strain supplements tRNAs with codons AUA, AGG, AGA, CUA, CCC, and GGA through a compatible * resistant plasmid. In this way, the Rosetta strain achieved the translation of "*". In general, due to the frequency limitation of codon usage in Escherichia coli, eukaryotic protein expression is often difficult. TRNA genes are driven by their natural promoters.

Cellregen can provideRosetta (DE3) Escherichia coli host bacteriumGlycerol bacteria or puncture bacteria

Product Catalog

Item Number	Product Name	Specifications	Catalog price
PB0911	Rosetta(DE3) glycerol stock	0.5 mL	850
PB0912	Rosetta(DE3) Puncture bacteria	branch	800

Note:

1. Glycerol bacteria: (1) Transportation method: transportation below 0 ℃ (2) Storage method: -80 ℃ frozen storage, regular (1 year) rejuvenation

2. Puncture bacteria: (1) Transportation method: 2-8 ℃ transportation(2) Storage method: stored at 4 ℃ for 1 week

Serruicheng provides multiple host bacteria for E. coli expression. For more favorable prices, please consult customer service