RNA UltraSense micro virus qRT PCR premix

useRNA UltraSense™one-step methodqRT-PCRPre mixed liquid, you will receive:

>high sensitivity

>High yield and high specificity

> RNAExcellent amplification effect of secondary structure

Ultra sensitive real-timeRT-PCR

RNA UltraSense™One Step QuantificationRT-PCRThe system is designed specifically forRNADesigned for amplification and real-time quantitative detection of viruses and ultra-low abundance transcripts. Optimized ultra concentrated premix combinedSuperScript™IIIReverse transcriptase（RT）AndPlatinum®Taq DNAPolymerase can provide higher primer specificity, higher amplification efficiency, and a wider detection dynamic range.

Optimized enzyme mixture with*Performance of

RNA UltraSense™one-step methodqRT-PCRThe premix combines two cutting-edge enzymes in the industry:SuperScript™IIIReverse transcriptase（RT）Yes, it isSuperScript™II RTThe point mutant,RNase HReduced activity. It has a longer half-life (in50°Cdown220Minutes) and higher thermal stability, which can provide highercDNAYield, amplificationRNAHigher success rate of secondary structures and higher specificity for gene specific primers.

Platinum®Taq DNAPolymerase provides antibody mediated hot start technology to reduce false starts and non-specific amplification, therebyPCRImprove specificity and sensitivity in the steps. by combiningSuperScript™III RTandPlatinum®Taq DNAPolymerase,RNA UltraSenseone-step methodqRT-PCRPre mixed liquid provides real-time solutions forqRT-PCRHighly sensitive and specific reaction system.

Ultra concentrated reagent for low abundanceRNASensitive amplification

RNA UltraSense™one-step methodqRT-PCRPre mixed solutions are particularly useful for studying rare transcripts or amplifying diluted templates.5X qRT-PCRThe reaction mixture can be added with about70%Samples of volume, compared to othersqRT-PCRThe premix has been concentrated2.5Twice. This higher concentration formula can be used for low concentration and/or large volume formulationsRNASamples provide greater flexibility. as low as25 pg / µlThe overallRNACan be detected, andPCREfficiency can be achieved97%.

Even with a large number ofRNASecondary structures can also be successfully amplified

Due to a large amount ofRNAThe existence of secondary structures, especially virusesRNAIt may be particularly difficult to amplify.RNA UltraSense™one-step methodqRT-PCRThe premix contains proprietary mixed enzymes that can promote the interaction between primers and templates under these difficult conditions, thereby improving performance. In addition,SuperScript™III RTHigh thermal stability (up to)60°CImplemented at a certain temperaturecDNASynthesis can enhance the richness ofRNAThe success rate of secondary structural targets.