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Shanghai Jinhao Scientific Instrument Co., Ltd
PSS NOVEMA gel column
NegotiableUpdate on 04/24
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Overview
PSS NovemaTM column: used for GPC analysis of water-soluble polymers (including cationic polymers)
Product Details
PSS NovemaTM Column: Used for water-soluble polymers（Including cationic polymers)The GPCanalysis
Scope of application
l Analysis of cationic polymers, anionic polymers, and neutral polymers in aqueous phase
l Quaternary ammonium polymers in the paper industry, flotation methods in the mining industry, flocculants in the leather and wastewater purification industries (FePO)4Coagulants, quaternary ammonium flotation agents such as DADMAC, and polyvinylpyridine in acidic mobile phase.
l Yin polymer, sulfonated polystyrene, sulfonated lignin, poly (methyl methacrylate), carboxylic acid modified polysaccharides.
l Proteins, enzymes, gelatin, etc
l Neutral polymers: polysaccharides, polyethylene oxide, polyethylene glycol, water-soluble starch and its derivatives.
l Can be applied to mixed systems of aqueous phase, water and acetonitrile, methanol or trifluoroacetic acid, or buffered aqueous solutions.
Not applicable scope
Avoid dry GPC bed and buffer precipitation on the column, which may cause a decrease in column performance. Do not let the column temperature drop below the freezing point of the mobile phase.
Key Features
l Durability: PPS NovemaTMThe column is made of very stable materials, with no measurable performance degradation under * use.
l Stability: The small hole column can withstand a pressure of up to 150 bar, while the large hole column can withstand a pressure of 45 bar, and can withstand temperatures from room temperature to 80 ° C0C is used (to avoid boiling of the mobile phase inside the column) and is stable between pH 1.5-12.
NovemaTMColumn is a universal material designed specifically for the analysis of cationic polymers in aqueous phase, and can also be used for the analysis of anionic polymers and neutral polymers in aqueous phase. There are four types of columns: analytical column, preparation column, micro meridian column, and high-speed column, with a particle size of 10 μ m.
Separation range
Separation range
PSS NovemaTM10µ 30O100D－3×104 D
PSS NovemaTM10µ 300100D－2×105 D
PSS NovemaTM10μ 1000 103D－1×106D
PSS NovemaTM10μ 3000Å 103D－>1.6×106D
PSS NovemaTM10μ 10000 5×103D－>5×106D
PSS NovemaTM10 μ linear500D－5×105 D
Table 7 PSS NovemaTMColumn performance indicators
| Molecular weight range | 100D to 5 × 106D |
| Zui High Pressure | 150—45bar, Depending on the aperture |
| Zui high temperature | 80℃ |
| Maximum flow rate (8mm i.D.) | 3ml/min |
| particle size | 10μm |
| Common mobile phases | Water, water/methanol, water/acetonitrile, buffered aqueous solution, water/trifluoroacetic acid |
Performance examples
Figure 7.1 GPC analysis of a complex mouse antibody against human lgG,
1.0ml/min, Mobile phase 0.0667MNaKHPO4Buffer solution, pH 6.8,
PSS NovemaTMColumn 8 × 300mm, injection volume 20 μ l, sample concentration 5mg/ml, detector UV230nm+RI
Figure 7.2 Using PSS NovemaTMColumn measured amylopectin calibration curve, molecular weight range 1600000 to 180D, 8 × 300mm, pore size 30 Å, 300 Å, 1000 Å, 3000 Å, 10000 Å (corresponding to low to high curve arrangement), mobile phase 0.05% NaNO3Water solution, injection volume 20 μ l, sample concentration 5mg/ml, flow rate 1ml/min, detection instrument RI
