The N2000 chromatography workstation chromatography signal acquisition card is the bridge between the chromatograph and the computer. The acquisition card itself contains a microcontroller processing system, placed separately in a shielded box, and has an independent external power supply. It is equipped with an imported 16/24 (optional) high-precision A/D (analog-to-digital) conversion chip (including PGA programmable amplifier, Gaussian low-pass filter, zero point, full scale, background, offset and other automatic correction functions), with a resolution of+-1um (ensuring full range linearity)

(1) Temperature drift automatic zero calibration function: With the continuous development of computer technology, the integration of chips is increasing, and the operating speed is accelerating, the temperature of computer chassis is getting higher and higher. With the temperature drift automatic zero calibration function, the acquisition card is not affected by external and internal temperature changes, ensuring the reproducibility of chromatographic analysis.

(2) Automatic calibration function for data errors: Utilizing advanced dynamic memory to continuously record and accumulate error data, automatic calibration is performed during data acquisition to minimize the impact of errors as much as possible

The N2000 chromatography workstation hardware can be connected to general chromatography instruments such as Shimadzu, Agilent, WATERS, etc. It is a universal chromatography data workstation. Widely used in various fields such as university research, chemical analysis, environmental testing, pharmaceutical inspection, food safety, etc., liquid chromatography analysis is highly favored by users.

Liquid phase detection application

Amoxicillin High Performance Liquid Chromatography (HPLC) Detection Scheme

-Sample processing procedure-

Accurately weigh about 25mg of the test sample and place it in a 50ml volumetric flask. Dissolve it in mobile phase and dilute it quantitatively to the mark. Shake well and filter through a needle filter to obtain the sample

-Chromatographic conditions-

Chromatographic column: Vertex C18:250mm × 4.6mm × 5 μ m

Mobile phase: Mobile phase A: Mobile phase B=2.5:97.5

Preparation method: Mobile phase A: Acetonitrile

Mobile phase B: 0.05mol/L potassium dihydrogen phosphate solution (adjusted to pH 5.0 with 2mol/L potassium hydroxide solution)

Wavelength: 254nm

Flow rate: 1.0 mL/min

Injection volume: 20ul

Instrument configuration:Please click to view the image details

	501Plus high-pressure constant current infusion pumpProvides solvent and compression factor libraries, which can automatically perform solvent compensation......		501Plus UV detectorThe original imported deuterium lamp used has a stable light source, extremely low baseline noise and drift. In addition, there is a tungsten lamp option, and the deuterium lamp has a warranty life of 1000 hours......		7725i injection valveIt is currently the most advanced HPLC sample injection valve. By using quantitative ring full filling and partial filling methods, sample volumes ranging from 1 μ l to 5ml can be processed.
	Solvent filterMade of ultra hard high-quality glass material, with uniform wall thickness, no bubbles, beautiful appearance, suitable for sterilization under high temperature and high pressure, good sealing performance, fast flow rate, and standard grinding mouth.		Kromastar chromatographic columnOur company has selected B-type ultra-high purity fully porous silicone gel (purity>99.999%) as the matrix......		N2010 Chromatographic Data WorkstationIt is a two in one chromatographic data workstation launched by Zhejiang University Zhida. Multiple user choices and high cost-effectiveness.

Hardware:

1. Dual channel, external type.

2. Output -5mv-1.7v.

3. The input impedance is greater than 10 megaohms.

4. Dynamic range of 107, linearity ± 0.1%.

5. Sampling frequencies are 10, 25, and 50 times per second.

6. RS232 communication method, key remote control, keyboard shortcut keys or mouse menu can also be used

Software:

1. Automatically identify solvent peaks, tailing peaks, sawtooth peaks, and front and back shoulder peaks.

2. Automatically adjust parameters (peak width, slope) during the analysis process.

3. Automatic baseline tracking and automatic classification of chromatographic peak types; Peak rise and fall points.

4. Powerful manual integration function, peak addition and subtraction, peak baseline adjustment, and cutting method adjustment.

5. Integral sensitivity of 1 microvolt · s, minimum resolution of 0.1 microvolts, and minimum peak width of 0.1 seconds.

6. The data results can be shared with software such as Microsoft Excel and Word, and the data spectra can be shared with image software such as Photoshop \ CrelDRAW.

7. Data reanalysis function, archiving.

8. Quantitative calculation methods: normalization method, calibration normalization method, internal standard method, grouping method, external standard method, index method.

Liquid phase detection application

Amoxicillin High Performance Liquid Chromatography (HPLC) Detection Scheme

-Sample processing procedure-

Accurately weigh about 25mg of the test sample and place it in a 50ml volumetric flask. Dissolve it in mobile phase and dilute it quantitatively to the mark. Shake well and filter through a needle filter to obtain the sample

-Chromatographic conditions-

Chromatographic column: Vertex C18:250mm × 4.6mm × 5 μ m

Mobile phase: Mobile phase A: Mobile phase B=2.5:97.5

Preparation method: Mobile phase A: Acetonitrile

Mobile phase B: 0.05mol/L potassium dihydrogen phosphate solution (adjusted to pH 5.0 with 2mol/L potassium hydroxide solution)

Wavelength: 254nm

Flow rate: 1.0 mL/min

Injection volume: 20ul

Instrument configuration:Please click to view the image details

	501Plus high-pressure constant current infusion pumpProvides solvent and compression factor libraries, which can automatically perform solvent compensation......		501Plus UV detectorThe original imported deuterium lamp used has a stable light source, extremely low baseline noise and drift. In addition, there is a tungsten lamp option, and the deuterium lamp has a warranty life of 1000 hours......		7725i injection valveIt is currently the most advanced HPLC sample injection valve. By using quantitative ring full filling and partial filling methods, sample volumes ranging from 1 μ l to 5ml can be processed.
	Solvent filterMade of ultra hard high-quality glass material, with uniform wall thickness, no bubbles, beautiful appearance, suitable for sterilization under high temperature and high pressure, good sealing performance, fast flow rate, and standard grinding mouth.		Kromastar chromatographic columnOur company has selected B-type ultra-high purity fully porous silicone gel (purity>99.999%) as the matrix......		N2010 Chromatographic Data WorkstationIt is a two in one chromatographic data workstation launched by Zhejiang University Zhida. Multiple user choices and high cost-effectiveness.

1. N2000 chromatography workstation version 6.1 has changed the algorithm of the data, making the online preview and offline preview data consistent, avoiding industry regulatory authorities' doubts about the authenticity of the data.

The N2000 chromatography workstation version 6.1 has powerful printing capabilities. Compatible with various printers, with good continuous printing results.

The N2000 chromatography workstation version 6.1 clearly indicates the "injection time" in the report, avoiding disputes among users regarding the experimental time.

The N2000 chromatography workstation version 6.1 does not display dialog boxes such as Access violation at address 00553286, Read of address 0000028 during or at the end of the sampling process, which results in the inability to save the sampling results and greatly consumes time.

In order to combat rampant piracy in the market and protect the interests of users, N2000 chromatography workstation version 6.1 adopts encryption dog technology. Without using a dongle, the software cannot be opened.

instructions:

1. After installing the software of N2000 chromatography workstation version 6.1 according to the instructions on pages 11-13, copy the green upgrade patch of version 6.1 from the USB drive to the computer and it can be used.

2. An encryption dongle must be inserted during use.

Liquid phase detection application

Amoxicillin High Performance Liquid Chromatography (HPLC) Detection Scheme

-Sample processing procedure-

Accurately weigh about 25mg of the test sample and place it in a 50ml volumetric flask. Dissolve it in mobile phase and dilute it quantitatively to the mark. Shake well and filter through a needle filter to obtain the sample

-Chromatographic conditions-

Chromatographic column: Vertex C18:250mm × 4.6mm × 5 μ m

Mobile phase: Mobile phase A: Mobile phase B=2.5:97.5

Preparation method: Mobile phase A: Acetonitrile

Mobile phase B: 0.05mol/L potassium dihydrogen phosphate solution (adjusted to pH 5.0 with 2mol/L potassium hydroxide solution)

Wavelength: 254nm

Flow rate: 1.0 mL/min

Injection volume: 20ul

Instrument configuration:Please click to view the image details

	501Plus high-pressure constant current infusion pumpProvides solvent and compression factor libraries, which can automatically perform solvent compensation......		501Plus UV detectorThe original imported deuterium lamp used has a stable light source, extremely low baseline noise and drift. In addition, there is a tungsten lamp option, and the deuterium lamp has a warranty life of 1000 hours......		7725i injection valveIt is currently the most advanced HPLC sample injection valve. By using quantitative ring full filling and partial filling methods, sample volumes ranging from 1 μ l to 5ml can be processed.
	Solvent filterMade of ultra hard high-quality glass material, with uniform wall thickness, no bubbles, beautiful appearance, suitable for sterilization under high temperature and high pressure, good sealing performance, fast flow rate, and standard grinding mouth.		Kromastar chromatographic columnOur company has selected B-type ultra-high purity fully porous silicone gel (purity>99.999%) as the matrix......		N2010 Chromatographic Data WorkstationIt is a two in one chromatographic data workstation launched by Zhejiang University Zhida. Multiple user choices and high cost-effectiveness.