GE affinity chromatography antibody purification pre loaded column (17-5498-54)

GE affinity chromatography antibody purification pre loaded column (17-5498-54)
Pre loaded column for affinity chromatography antibody purification
HiTrap MabSelect PrismA, 5×5mL
Product Code: 17549854
Brand | Manufacturer: GE
Provide tools for purifying monoclonal and polyclonal antibodies from different sources.
The basis of antibody affinity purification is the high affinity and specificity of protein G and protein A for the Fc region of IgG from different species. Fixing protein G or protein A on chromatography media of different matrices provides a method for rapidly separating IgG and IgG subclasses from ascites, cell culture supernatant, and serum.

At the same time, we also have chromatography media for recombinant protein L ligands for the purification of antibodies and antibody fragments. Protein L has a strong affinity for the variable region of kappa light chains and can be widely used to capture antibody fragments such as Fab fragments, single chain variable region fragments (scFv), and binding domain antibodies (Dabs).

GE affinity chromatography packing is a technique for separation based on reversible interactions between target proteins and specific ligands on the guiding matrix. This interaction can be either specific (such as the binding of antibodies to protein A or protein G) or non-specific (such as the binding of tagged proteins to metal ions).
There are many types of biological interactions applied in affinity chromatography, such as ligand substrate, protein protein, nucleic acid protein, and so on. The affinity chromatography process generally includes four stages: equilibrium, sample loading, cleaning, and elution. Balance mainly refers to balancing the column in an environment that is conducive to the binding of the sample and filler, and then loading the sample. Elution can be specific (such as competitive ligand molecules) or non-specific (such as altering pH or ionic strength). Affinity chromatography has the advantage of high selectivity, and usually a single affinity chromatography column can obtain high-purity products. However, when higher purity is required, one or more purification steps need to be added, such as purification after gel filtration chromatography.
Affinity chromatography packing can be used for purifying tag proteins, antibodies, or specific molecules. Affinity chromatography can also be used to remove specific pollutants.
General Electric Medical Group recently launched a new affinity chromatography medium, Capto L, suitable for capturing antibodies and antibody fragments. It combines a rigid high flow agarose matrix with a recombinant protein L ligand that binds to immunoglobulin, which has strong affinity for the variable region on the antibody K light chain. Therefore, Capto L is suitable for capturing various antibody fragments, such as Fab, single chain antibodies (scFv), and domain antibodies.
Compared with ion exchange and hydrophobic interaction chromatography, the affinity chromatography capture step has a very stable and universal processing procedure. The optimization limit required for Capto L purification is small, and a high purity can be obtained in one step. This significantly saves costs, time, and efficiency during the production process. Compared with other methods, the high selectivity of the affinity chromatography capture step will help reduce the subsequent fine purification steps, and fewer selective purification media will be used in production, which can also benefit from it.

With its highly dynamic binding ability and high selectivity, Capto L improves the economy of the process and the flexibility of process design. Capto L is a BioProcess chromatography medium, which means that this medium is designed and developed for large-scale production of biopharmaceuticals and has received relevant supporting documents. These supports include confirmed production methods, reliable media supply, safe and easy to operate management support documents (RSFs) that assist in process validation, and documents submitted to regulatory authorities.

Capto L is available in large packaging and pre packaged columns, which can accelerate the screening and optimization of binding and elution conditions.
Important performance characteristics include:
• High dynamic binding ability and wide range of antibody fragment selectivity
• Ensured a solution for purifying K light chain antibody fragments
• Allow rigid substrate substrates with high flow rates, high productivity, and low ligand leakage
• BioProcess media with management support and assurance for commercial production supply