French Komagai B Streptococcus chromogenic medium

CHROMagar™ Komagai, FranceBStreptococcus mutans chromogenic medium 1500yuan/1000ml

This chromogenic medium is used for separation and identificationBStreptococcus mutans.

1、 Composition

One bottle of Komajia Bacillus cereus basic culture medium and supplementS1andS2One bottle each.

Basic dry powder and supplementsS1Stored in15-30Supplement in ℃ environmentS2Stored in2-8In a temperature environment.

2、 Ingredients（g/L）

Agar: 15.0 Protein peptone and yeast extract 20.0 Salt content 7.5 pigment 4.5

pH：7.3±0.2

3、 Operation:

⊙ Basic dry powder+SupplementsS1

Add the basic dry powder from the bottle1000mlDistilled water (can be adjusted as needed)44.7g/LMix and dissolve the mixture in a larger or smaller proportion, and then follow the instructions2g/LAdd supplements in proportionS1Stir until the agar dissolves. Conventional high-pressure sterilization（121℃,15min）Then cool in a water bath until45-50℃, keep stirring.

⊙ SupplementS2

Take a transparent container and use it10mlDissolve in distilled water0.25gSupplementsS2According to25mg/mlthe proportionExpand or shrink）Quickly stir evenly with a magnetic stirrer to dissolve it. After filtering and sterilizing, follow the steps10ml/LAdd the prepared basic dry powder in proportion+SupplementsS1Gently mix the mixture in the liquid, then pour it into a sterile culture dish to solidify.

This finished tablet can be stored at room temperature for one day; Can be stored in the refrigerator for two weeks（2-8℃, away from light and dry.

3、 Result

★ The used culture medium needs to be121Sterilization at least at ℃20Only minutes are allowed to be discarded according to relevant regulations

Note:

1.Incubation in carbon dioxide may lead to false positives

2.a very small numberBStreptococcus may require additional extension24An hour of cultivation time is required to achieve satisfactory colony size

3.someATheCTheGStreptococcus may appear purple in color

4.The final identification requires additional testing experiments