Four channel 48 well PCR instrument

1、 Basic performance

Dimensions: 410 × 320 × 196mm

Weight: 11kg

Power supply: 220V

Power: Working power 900W

Operating System: Optional Windows computer (Win7 or above)

System language: Real time switching between Chinese and English

Indicator lights: standby breathing light, temperature rise and fall yellow green switching indicator light

Communication interface: Square USB

2、 Environmental requirements

Operating environment temperature: 18~35 ℃

Operating environment relative humidity: 0%~85%

Transportation and storage temperature: -20~55 ℃

3、 System performance

Sample size: 48 wells x 0.2mL

Sample volume: 20~120uL

Applicable consumables: 0.2mL single tube, 8 × 0.2mL eight connected tube, 48 well PCR plate

Temperature control range: 4-99 ℃

Temperature control method: High performance imported Peltier module

Gradient temperature control: 6 modules gradient temperature control, temperature difference support 25 ℃

Heating rate: 7 ℃/s

Cooling rate: 4.5 ℃/s

Temperature control accuracy: ± 0.1 ℃

Temperature uniformity: ± 0.25 ℃

Temperature accuracy: ± 0.1 ℃

Operation function: preset projects and analysis parameters, easy and convenient to operate

Report function: Support custom report modules, print reports by item for 48 holes

Analysis function: With multiple qPCR data analysis capabilities, it can simultaneously detect 4 fluorescence channels

Analysis algorithms: amplification curve, standard curve quantification, melting curve analysis, SNP genotyping

Data export: The experimental results can be directly exported in the form of graphics and tables. The detailed data report includes experimental conditions, data coordinates and tables, and data analysis parameters

TouchDown function: supports time and temperature increasing or decreasing modes

Software Upgrade: Analyze Software Upgrades

4、 Fluorescence detection system performance

Light source: 4 long-life LED light sources with different filters, which can specifically and efficiently excite fluorescent molecules in different excitation bands

Detector: 4 photodiodes with different filters for specific detection of different emission light bands

Excitation and detection propagation medium: Fluorescent molecule imported high-sensitivity sensor, each well can simultaneously detect 4 target factors of excitation and detection propagation medium, high-temperature resistant professional glass core optical fiber

Excitation light wavelengths: 46-480nm, 520-545nm, 575-595nm, 600-620nm

Detection light wavelengths: 505~525nm, 555~575nm, 600~620nm, 660~680nm

兼容染料： FAM/SYBR Green/Eva Green，VIC/HEX/TET/CY3/JOE，ROX/Texas Red，CY5/CY5.5