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Beijing Punard Technology Co., Ltd
CHROMagar™ French Komajia KPC color development medium
NegotiableUpdate on 01/21
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Overview
CHROMagar One bottle of French Komagai KPC chromogenic medium for detecting carbapenem resistant bacteria (KPC) and one bottle of Komagai urinary tract microbiota chromogenic medium and KPC supplement
Product Details
CHROMagar™ Komagai, FranceKPCChromogenic medium
Used for carbapenem resistant bacteria（KPC）The detection
group divide
Komajia urinary tract microbiota chromogenic medium andKPCOne bottle of each supplement.
Basic culture medium: Agar15.0 g/L Protein peptone and yeast powder 17.0 g/L pigment1.0 g/L
increase supplement Agent:0.4 g/L pHvalue 7.0
operation
1Basic culture medium: Take dry powder from the bottle33.0gdissolve in1000mLIn a clean triangular flask of deionized water, stir thoroughly and mix well. You can also follow as needed33.0 g/LIncrease or decrease the proportion of the prepared culture medium.
2Heat up to100℃Stir continuously to dissolve it. If using a microwave oven for heating, the culture medium should be heated to boiling, immediately removed, gently shaken, and then placed in the microwave oven for heating. Observe the small bubbles turning into large bubbles until dissolved. Do not let the culture medium overflow. Sterilize the dissolved culture medium under high pressure（121℃，15min）.
3Supplements: Take supplements0.4gdissolve in10mlIn sterile deionized water, stir evenly or as needed according to specific requirements0.4 g/LIncrease or decrease the proportion of supplements prepared.Note: Stirring into a light yellow opaque homogeneous solution may take a few minutes. The prepared supplement solution must be used up on the same day and should not be stored or reused.
4Add the dissolved supplement to cool to45℃~50℃Localization of urethral microbiota in Komajia chromogenic culture mediumGently shake it evenly and prepare it into KomajiaKPCColor developing medium, pour into a petri dish to solidify, air dry for later use.
save The finished tablet can be stored at room temperature for one day or in a refrigerator2month（2℃~8℃, avoid light).
vaccinate
Marking or coating inoculation (plates stored in the refrigerator should be restored to room temperature before use)36
result
| Microorganisms（Microorganism） | Colony color |
| Carbapenem resistant Escherichia coli（CarbapenemR E.coli） | Deep pink to red |
| Carbapenem resistant Klebsiella, Escherichia coli, CitrobacterCarbapenemR KEC (Klebsiella, Enterobacter, Citrobacter) | Metallic Blue |
| Carbapenem resistant Pseudomonas aeruginosa（CarbapenemRPseudomonas） | Translucent, green pigment precipitate |
| Carbapenem resistant Acinetobacter baumannii（CarbapenemR Acinetobacter） | Creamy texture |
| Oligotrophic Pseudomonas aeruginosa（Stenotrophomones） | Colorless |
| Gram positive bacteriaGram(+) strains | inhibit |
| Carbapenem strains（CarbapenemSstrains） | inhibit |
| Yeast（Yeasts） | Most inhibition |
Performance and limitations
The final identification requires additional biochemical or immunological tests.
Storage conditions
Dry powder needs to be stored in15℃~30The supplement should be stored in a dry environment at ℃2℃~8In a temperature environment, use before the expiration date.
pollution treatment
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