CHROMagar™ French Komagai ESBL chromogenic medium

NegotiableUpdate on 01/21

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

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Overview

CHROMagar The French Komagai ESBL chromogenic medium is mainly used for the isolation and identification of drug-resistant strains producing extended spectrum β - lactase (ESBL), while inhibiting the growth of ampC resistant bacteria

Product Details

CHROMagar™ Komagai, FranceESBLChromogenic medium

Mainly used for producing extended spectrum β - lactases(ESBL)Isolation and identification of drug-resistant strains, while inhibiting those withampCThe growth of resistant bacteria.

group divide

Komajia urethral microbiota localization colorimetric culture medium andESBLOne bottle of each supplement.

Basic culture medium: Agar15.0 g/L Protein peptone and yeast powder 17.0 g/L pigment1.0 g/L

increase supplement Agent:0.57g/L

pHvalue 7.0

operation
1Basic culture medium: Take dry powder from the bottle33.0gdissolve in1000mLIn a clean triangular flask of deionized water, stir thoroughly and mix well. You can also follow as needed33.0 g/LIncrease or decrease the proportion of the prepared culture medium.

2Heat up to100℃Stir continuously to dissolve it. If using a microwave oven for heating, the culture medium should be heated to boiling, immediately removed, gently shaken, and then placed in the microwave oven for heating. Observe the small bubbles turning into large bubbles until dissolved. Do not let the culture medium overflow. Sterilize the dissolved culture medium under high pressure（121℃，15min）.

3Supplements: Take supplements0.57gdissolve in10mLIn sterile deionized water, stir evenly or as needed according to specific requirements0.57 g/LIncrease or decrease the proportion of supplements prepared.Note: Stirring into a light yellow opaque homogeneous solution may take a few minutes. The prepared supplement solution must be used up on the same day and should not be stored or reused.

4Add the dissolved supplement to cool to45℃~50℃Localization of urethral microbiota in Komajia chromogenic culture mediumGently shake it evenly and prepare it into KomajiaESBLColor developing medium, pour into a petri dish to solidify, air dry for later use.

save The finished tablet can be stored at room temperature for one day or in a refrigerator1A month（2℃~8℃Avoid light.

vaccinate

Marking or coating inoculation (plates stored in the refrigerator should be restored to room temperature before use)36

result

Microorganisms（Microorganism）	Colony color
ESBLEscherichia coli（ESBLE.coli）	Deep pink to red
ESBLKlebsiella, Escherichia coli, CitrobacterESBL KEC (Klebsiella,Enterobacter,Citrobacter)	Metallic Blue
ESBLProteus mirabilis（ESBLProteus）	Brown halo ring
ESBLAcinetobacter baumannii（ESBLAcinetobacter）	Creamy texture
ESBLPseudomonas （ESBLPseudomonas）	Translucent, green pigment precipitate
Oligotrophic Pseudomonas aeruginosa（Stenotrophomones）	Colorless
Gram positive bacteriaGram(+) strains	inhibit
Not producedESBLGram negative bacteria	inhibit
Yeast（Yeasts）	Most inhibition

Performance and limitations

The final identification requires additional biochemical or immunological tests.

Storage conditions

Basic dry powder needs to be stored in15℃~30The supplement should be stored in a dry environment at ℃2℃~8In a temperature environment, use before the expiration date.

pollution treatment

The used culture medium needs to be121Sterilization at least at ℃20Only minutes are allowed to be discarded according to relevant regulations.

Attachment: For external diagnostic use, the product must be operated by professional personnel.