CHROMagar™ Chromogenic Culture Medium for Shigella Coli from France

CHROMagar™ Komagai, FranceShigella Chromogenic Medium

This medium is used for selective isolation of Shigella.

1. Packing Specification

   Fundamentals of Shigella Chromogenic Culture Medium 64.7g/L

   additive 1bottle/L

   PH 6.7±0.2（25℃）

   Classic formulas can be adjusted or supplemented as needed.

2. operation

1. 取64.7gDry powder dissolved in970mlDistilled water, mix, stir and boil until fully dissolved, cool to50Keep warm at around ℃ for future use.

2. use30mlDissolve in distilled water1bottle/LThe additive is thoroughly shaken and dissolved to a light yellow solution, and then used0.45Mmor0.22MmFilter and sterilize with a filter membrane, add sterile culture medium base from the previous step, mix well, and pour onto a plate.

   Note: Weigh and add culture medium, additives, etc. according to the proportion. After dissolving and filtering the additives, they can be stored in the dark and refrigerated3A month.

1. store

   Basic dry powder needs to be stored in15-30℃, in a cool and dry environment.

   Additives in2-8Store in a dark environment at ℃.

2. result

   After pre culture, take the bacterial growth solution and inoculate it with chromogenic medium,36±1℃ cultivation22±2h.

Note: Supplementary experiments must be conducted to confirm the results.

The used culture medium needs to be121Sterilization at least at ℃20Only minutes are allowed to be discarded according to relevant regulations.