The basic structure of the instrument is the real-time fluorescence quantitative PCR system, which is an instrument for real-time detection of reactions. It mainly consists of a gene amplification thermal cycle system, a fluorescence real-time detection system, a microcircuit control system, a computer, and application software. Two core functional modules are: thermal cycling system and real-time fluorescence detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. Fluorescence detection ..












Basic structure of the instrument
The real-time fluorescence quantitative PCR system is an instrument for real-time detection of reactions, mainly composed of a gene amplification thermal cycle system, a fluorescence real-time detection system, a microcircuit control system, a computer, and application software. Two core functional modules are: thermal cycling system and real-time fluorescence detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, optical signal transmission components, fluorescence detection components, and control systems.
Instrument technical specifications
The symbol "//" indicates that the national standard currently does not define this indicator or mandatory requirement.
indicator name |
H160 |
H320 |
H480 |
appearance |
Overall dimensions |
235mm * 385mm * 175mm (width * depth * height) |
weight |
5.6kg |
5.7kg |
5.8Kg |
Electrical parameters |
~220V/50Hz,255W |
data interface |
USB 2.0 * 2 (two on the right) |
environmental parameters |
Operating conditions |
Temperature: 10-30 ℃, humidity: 20%~80% |
Transportation and storage conditions |
Temperature: -20~55 ℃, humidity: 20%~80% |
altitude |
<2500 meters |
Noise level |
A-weighted,<60dB |
Sample parameters |
sample size |
16*0.2mL |
32*0.2mL |
48*0.2mL |
Test tube type |
Single tube, eight row test tube |
Sample volume |
15-100uL |
temperature characteristics |
Heating/cooling method |
Semiconductor heating/cooling |
temperature range |
4℃-99℃ |
heating rate |
≥3.5℃/s |
Average heating rate |
≥2.5℃/s |
降温速率 |
≥3.5℃/s |
Average cooling rate |
≥2.5℃/s |
temperature control accuracy |
≤±0.01℃ |
Temperature accuracy |
≤±0.1℃ |
temperature uniformity |
≤±0.3℃ |
optical properties |
Number of fluorescence detection channels |
4通道 |
light-emitting device |
high-brightness LED |
Daylighting device |
High sensitivity, high signal-to-noise ratio photodiode |
Adapt probes or dyes |
Channel: 470/520 FAM, SYBR Green |
Second channel: 530/570 HEX, JOE, VIC |
Third channel: 580/610 ROX, CY3.5, Texas Red |
Fourth channel: 630/670 CY5 |
detection sensitivity |
1 copy |
Linear detection range |
100~1010 copies |
Linear correlation coefficient |
≥0.999 |
Channel crosstalk |
No crosstalk |
Detecting repeatability |
≤1.0% |
Main application areas of the instrument
◆ Foodborne pathogen detection
◆ Food safety
◆ Animal and plant origin testing
◆ Plant Science and Agricultural Biotechnology
◆ Gene mutations and polymorphisms
◆ Animal diseases
◆ Basic scientific research
◆ Water monitoring
◆ Pathogenic nucleic acid testing
◆ Transgenic testing
◆ Animal epidemic monitoring
◆ Food hygiene quarantine
◆ Customs import and export quarantine