enh001 kerafast

NegotiableUpdate on 01/21

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Kerafast Beijing Agent Kerafast North China Agent, Kerafast East China Agent, Kerafast South China Agent, Kerafast Central China Agent, Kerafast Agent, Kerafast Agent, Kerafast Agent Beijing Huaxin Kangxin Biotechnology Co., Ltd. Kerafast products, Kerafast spot, Kerafast various series of products, kerafast

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Kerafast spot, enh001 kerafast*

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Enha001 kerafast anti DNA-RNA hybridization [S9.6] antibody

This mouse monoclonal antibody is produced against DNA-RNA antigens synthesized by the Φ X174 bacteriophage and can recognize various lengths of RNA-DNAza species.

emphasize

It is very useful in detecting R circuits for DNA RNAza, which has high specificity and affinity and does not cross react with single stranded DNA or double stranded DNA. For AU rich double stranded RNA, smaller cross reactions have been observed (about 5 times less). High affinity binding was demonstrated for hybrids with lengths of 8, 10, 15, and 23 base pairs

Available reorganized versions:

Production of selected isotopes/formats using antibody recombinant platform with variable region (i.e. specificity) from hybridoma S9.6: mouse IgG2a, rabbit IgG, mouse Fab fragment, goat IgG

DNA-RNAza species are a natural phenomenon in eukaryotic cells, and their levels are elevated at sites with high transcriptional activity, such as during transcription initiation, inhibition, and elongation. Due to the impact of RNA DNAza species on genomic instability, S9.6 antibodies are useful reagents for studying the consequences of R loops and damage formed by these za species during DNA replication or other cellular processes. In addition, S9.6 antibodies can effectively recognize RNA hybridization used for microarray research.

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Catalog number	product	size	usability	quantity
ENH001	resistDNA-RNAhybridization[S9.6]Antibodies,100ug	100microgram	in stock	ENH001agent spot
ENH002	resistDNA-RNAhybridization[S9.6]Antibodies,1mg（10x100ug）	1Milligrams（10x100ug）	in stock	ENH001agent spot
Kf-Ab01137-2.0	Recombinant resistance[S9.6], miceIgG2a，Mr，100ug	100microgram	in stock	ENH001agent spot
Kf-Ab01137-23.0	Recombinant resistance[S9.6]The RabbitIgG，100ug	100microgram	in stock	ENH001agent spot
Kf-Ab01137-1.6	Recombinant resistance[S9.6], miceFabFragment,His-Tagged，Mr，100ug	100microgram	in stock	ENH001agent spot
Kf-Ab01137-24.1	Recombinant resistance[S9.6]goatIgG，100ug	100microgram	in stock	ENH001agent spot

Enh001 Kerafast spot, enh001 Kerafast agent, enh001 kerafast*

technical indicators

Product category:	antibody
Antigen:	S9.6ΦX174Phage synthesis of DNA-RNA antigens
同型：	Mouse IgG2a, Rabbit IgG, Mouse Fab, Goat IgG
Clonality:	Monoclonal
Fusion tags:	The mouse Fab version includes His tag
Clone Name:	S9.6
Reactivity:	Highly specific and affinity for DNA/RNA hybrids and other A-type nucleic acid hybrids
immunogen	ΦX174DNA/RNA synthesized by bacteriophages
Immune species:	BALB / cmouse
Complementary position:	DYYGSRWFDY(Suggestion)
Purification method:	Protein A/G
Buffer:	ENHOO1：0.1MSodium phosphate, pH7.4，0.15MNaCl, 0.05% (w/v) azide NA Ab01137-：PBS and 0.02% Proclin 300
Tested applications:	Dot blot analysis:0.2 µg / mL. Affinity binding assay:Clone S9.6 binds equally to DNA-RNA heterozygotes and poly (I) - poly (dC), but to achieve similar levels, 100 fold higher levels of poly (A) - poly (dT) are required. Binding. Single stranded DNA, double stranded DNA, RNA, and ribosomal RNA were not cloned and bound to S9.6 (Boguslawski, SJ et al, （1986）.J.Immunol Methods.89（1）：123-130）。 Chromatin immunoprecipitation (ChIP) analysis:In HeLa cells, a representative batch detected an increase in DNA RNAza species on four actively transcribed genes in shRNA mediated knockdown of BRCA1 or BRCA2 rather than PCID2 or RAD51 (Bhatia, V., et al. (2014) Nature 511 (7509): 362-365). Chromatin immunoprecipitation (ChIP) analysis:A typical batch was detected using HeLa chromatin preparation to form an R loop on the β - actin gene. The treatment of chromatin preparations by RNase H prevented the cloning of S9.6 immunoprecipitation target chromatin fragments (Skourti Stathaki, K., et al. (2011)). Mol。 Cell。 42 (6): 794-805). Chromatin immunoprecipitation sequencing (ChIP seq) analysis:A representative batch was analyzed using ChIP seq to determine the genome-wide distribution of DNA-RNAza species in germinating yeast (El Hage, A. et al. (2014)). PLoSGenet。 10 (10): e1004716). Immunocytochemical analysis:Immunolocalization of nuclear R-rings in representative batches was performed by fluorescence immunohistochemistry staining of H1 human embryonic stem cells (hESCs) fixed with methanol and HeLa cells fixed with formaldehyde (Bhatia, V., et al., 2014). Nature。 511（7509）：362- 365； Ginno, PA et al. (2012). Mol. Cell. 45 (6): 814-825). Immunoprecipitation analysis:Representative batches transcribed R-loop substrates (DNA RNAza species) in vitro, but without immunoprecipitation of double stranded DNA (dsDNA) (Gino, PA et al. (2012)). Molecular Cell 45 (6): 814-825). Please also refer to: S9.6Application publications
storage	-20°C(Avoid repeated freeze-thaw cycles)
shipment	ice pack

Enh001 Kerafast spot, enh001 Kerafast agent, enh001 kerafast*

Anti DNA-RNA hybridization [S9.6] antibody

Amplification of Anti DNA-RNA Hybridization [S9.6] Antibody

This mouse monoclonal antibody is produced against DNA-RNA antigens synthesized by the Φ X174 bacteriophage and can recognize various lengths of RNA-DNAza species.

emphasize

Available reorganized versions:

Production of selected isotopes/formats using antibody recombinant platform with variable region (i.e. specificity) from hybridoma S9.6: mouse IgG2a, rabbit IgG, mouse Fab fragment, goat IgG

Beijing Huaxin Kangxin Biotechnology Co., Ltd. focuses on the field of life sciences and is composed of a technical and management team with rich industry experience. Its main products include biochemical reagents, instruments, experimental consumables, etc. A wide range of products in the radiation field, including reagents, consumables, instruments, etc. Kerafast Beijing Agent Kerafast North China Agent, Kerafast East China Agent, Kerafast South China Agent, Kerafast Central China Agent, Kerafast Agent, Kerafast Agent, Kerafast Agent Beijing Huaxin Kangxin Biotechnology Co., Ltd. Kerafast products, Kerafast spot, Kerafast various series products, Kerafast knowledge introduction, Kerafast technical services, Kerafast consulting services, Kerafast cooperation services, welcome to contact us for sincere cooperation and win-win cooperation.

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During the Kerafast event, all of the company's products are available at *. While you can buy high-quality products, you can also enjoy this discounted price. We hope to cooperate with you for a win-win situation. Kerafast has a large amount of stock available, ensuring delivery on the same day of purchase and delivery by SF the next day. We guarantee quality and quantity, and provide home service. Beijing Huaxin Kangxin is the agent of Kerafast. Your satisfaction and recognition will increase our confidence, further provide better services, and wholeheartedly cooperate for win-win results.

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