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Beckman Coulter MoFloAstrios EQAdopting the world's most advanced microbead free droplet delay detection technology, Inlisort II, can eliminate the traditional practice of using microbeads to achieve droplet delay. This can reduce the need to remove sterile samples when recalculating droplet delay time due to nozzle blockage, ensuring that the sterile state is not interrupted during the sorting process. In addition, Inlisort II can monitor and maintain the stability of droplet breakpoints to achieve uninterrupted sterile sorting, ensuring the integrity of sorting.
The dual forward angle scattering light detection technology can simultaneously measure the characteristics of scattered light signals on three orders of magnitude. Appropriate wavelength selection can expand the detection range of eFSC to 405-640 nm, enabling detection of both smaller and larger particles. With the help of this instrument, researchers can conduct research on a wide range of biological samples, from microsomes to macrophages, from astrocytes to xenografts, all of which can be analyzed and sorted.
The instrument can also be used for some non biological applications, such as agarose gel drops, gold nanoparticles or environmental particles; Or single-cell research, such as circulating tumor cells, new drug development, rare analysis, stem cells and nerve cells, as well as microbial ecology and physiology. |
MoFloAstrios EQ optical performance:
The dual path enhanced forward light scattering system (eFCS) can collect simultaneously.
Forward scattered light signal wavelength optional (excluding 355nm ultraviolet laser)
There are 7 types of eFCS masks available in S, M, and P categories, as well as customizable masks for customers to choose from. All masks can be freely combined in pairs.
7 Laser -7 Pinhole Excitation System, optional lasers include: 355nm, 405nm, 488nm, 532nm, 561nm, 592nm, 642nm.
All laser signals and scattered light are conducted through optical fibers (except for 355nm ultraviolet excitation light).
Zui can select signals containing 51 parameters within the scattered light signal (simultaneously collecting 32 parameter signals).
All lasers can be configured with independent side scattered light (SSC) collection channels (except for 355nm ultraviolet laser).
MoFlo Astrios EQ Liquid Flow System Performance:
Liquid flow pressure: 0-100 psi infinitely adjustable.
The liquid flow system is equipped with a 3-level filtration system, which effectively filters 99.999% of impurities larger than 0.1 μ m in the sheath fluid. Optional ultrafine particle filter can remove particles larger than 0.04 μ m. Air filters can filter particles larger than 0.01 μ m.
Teflon material pipeline, resistant to acid, alkali and high temperature, can be washed with various volumes. Wear resistance and aging resistance effectively eliminate sample residue.
The Smartsampler automatic sampling system supports 0.5 mL, 1.0 mL, 1.5 mL, 5 mL, 7 mL, 15 mL, and 50 mL sample tubes. And it has functions such as fully automatic cleaning, backwashing, and bubble removal. And it has temperature control function.
MoFloAstrios EQ electronic processing performance:
Droplet oscillation frequency: 200kHz.
Data scanning frequency: 100MHz.
Electronic dead time: 0 μ s.
Original data accuracy: 32-bit.
Lossless data amplification function: Up to 16 x lossless amplification of data images.
Single file storage capacity:>1000000000 Events.
MoFlo Astrios EQ automation performance:
The timed startup function allows for separate setting of liquid flow and laser activation.
Fully automatic QC and laser delay setting.
Inlisort II fully automatic droplet delay setting and sorting status maintenance function.
SortRescue automatic sorting protection function.
Automatic shutdown and cleaning program.
MoFlo Astrios EQ sorting performance:
Theoretical maximum sorting speed: 200000 cells/second.
Sorting purity:>99% (using a 70 μ m nozzle at a speed of 70000 per second, 60psi pressure, and purification sorting mode to sort samples with a target cell content of 1%).
Recovery rate:>90% of the theoretically expected sorted cells.
Sorting activity: Under different nozzles, sheath fluids, and pressures, the sorted cultured cells can begin to proliferate within a few days after sorting.
Effective acquisition speed:>100000 cells/second.
Effective sorting speed:>70000 cells/second.
CyClone (Clone Sorting):
Plate sorting: preset 6-1536 orifice plates, user-defined orifice plate types, with the highest sorting precision up to 250dpi.
Water bath temperature control, capable of heating or cooling (optional).
Tube sorting: the highest 6-way sorting.
2-channel sorting: receive samples in 1.5, 5, 15, and 50 mL tubes.
4-way sorting: 4 × 5 mL, 3x5 mL+1x50 mL sample tube reception.
6-way sorting: Received in 1.5 or 5 mL sample tubes.
Water bath temperature control, capable of heating or cooling (optional).
Glass slide sorting: preset up to 1536 dots, users can customize the dot matrix type, and the highest sorting precision can reach 250dpi.
InliSort II (fully automatic time delay and monitoring):
InliSort II can adjust droplet breakpoints and calculate time delays without the involvement of microspheres, and monitor and maintain the stability of droplet breakpoints throughout the process.
MoFlo Astrios EQ is not limited by instruments
Up to 51 detector sites, including 2 enhanced forward scattered light (eFSC) detectors and 49 photomultiplier tubes, with a maximum of 7 detectors per laser,
Each loaded laser is equipped with a precision optical component (POD), and each POD can accommodate up to 7 photomultiplier tubes (PMT)
In addition to fluorescence signals, any laser excited forward scattered light (FSC) and side scattered light (SSC) can be used as trigger signals
You can choose detectors at any position to detect your sample
Quickly replacing parts allows you to easily and quickly switch applications
*This product is only used for scientific research purposes and is not intended for clinical diagnostic processes.
MoFlo Astrios EQ frees itself from quartz cup sorting limitations
Reliable analysis of heterogeneous samples: There is no velocity difference between particles of different sizes; Eliminating the requirements for area/height ratio and laser time delay optimization
Maintain stable performance with less maintenance without worrying about quartz cup aging and generating fragmented signals
Based on the biological characteristics of the sample, the sorting conditions are determined. There are nozzle sizes of 70 and 100 microns * available for precise adaptation to any tissue derived cell. Inlisort II is used to automatically calculate and maintain droplet delay
MoFlo Astrios EQ Biosafety Design:
Sorting monitoring and rescue, automatic stop of sheath fluid flow, sample flow, deflection plate voltage and alarm in case of exceeding the biological safety level.
*Aerosol elimination system.
Removable sample tubing and injection needle.
High pressure sterilization nozzle, sheath tank, and waste liquid tank.
Pluggable sorting compartment door with a smooth sorting compartment design that avoids scratches.
Easy to clean sorting deflection plate and nozzle with quick disassembly design.
*If you need nozzles of other sizes, please have a business representative from Beckman Coulter Company
| | Laser configuration | | wavelength | power | Beam characteristics | | 355nm | 100mW | Elliptical | | 405nm | 55mW | Flat-Top | | 488nm | 150mW | Flat-Top | | 532nm | 150mW | Flat-Top | | 561nm | 200mW | Flat-Top | | 592nm | 200mW | Flat-Top | | 642nm | 100mW | Flat-Top | | | |
