Thermo liquid chromatography column

Monoclonal antibodies (MAbs) are therapeutic proteins, and this industry is rapidly developing. When developing recombinant monoclonal antibodies, rapid screening of cell culture samples and determination of IgG titers are required. People usually use affinity chromatography columns containing Protein A ligands to determine MAb concentration. Our MAbPac amp; amp; amp; amp; amp; trade; Protein A chromatography column is a high-performance liquid chromatography (HPLC) column used for rapid and accurate titer analysis, with a wide linear range and not easily overloaded.

　　Thermo Scientific ™ MAbPac ™ Protein A chromatographic columnConjugated with recombinant Protein A ligand:

Affinity chromatography is the most effective way to determine the concentration of IgG in cell culture media. Protein A chromatography column can specifically bind to the Fc region of IgG, thereby obtaining accurate concentration determination results.

Thermo Scientific ™ MAbPac ™ Protein A chromatographic columnInnovative non porous polymer fillers with hydrophilic surfaces:

The packing material has been specially designed with fast mass transfer characteristics, which can reduce the peak width of IgG and improve column efficiency. It can be used to determine extremely low concentration ranges (as low as 0.025 mg/mL).

The hydrophilic surface can minimize non-specific binding, ensuring that the chromatographic column only retains IgG protein, thereby improving the accuracy of MAb titer determination results. At the same time, due to the good specificity of the chromatographic column, there is no need for additional purification steps on the sample, which shortens the analysis time.

Low column pressure: It also has a lower column pressure at a flow rate of 2 mL/min

Using a flow rate of 2 mL/min, the analysis process can be completed within 2 minutes. Due to the frequent need to screen a large number of clones and cells during product development, high-throughput affinity chromatography columns are your ideal choice.

　　Thermo Scientific ™ MAbPac ™ Protein A chromatographic columnWide quantitative range: 0.025mg/mL to 5 mg/mL

• It can quickly determine various concentrations of IgG without the need for complex method optimization.