Stoelting standard brain stereotaxic device

Introduction

The standard series of Stoelting standard brain stereotaxic devices in the United StatesBrain stereotaxic deviceFor the stereotaxic localization of small animal brains, it is a reliable and multifunctional device. By positioning the instrument, it is possible to ensure the proper positioning of electrodes, microtubes, and other equipment during the experimental process.

The time tested U-shaped frame design, sturdy structure, and adapter that can be used with almost all experimental animals ensure that this product is the best choice for brain stereotaxic devices.

1．The ruler is easy to read All rulers are positioned to read from an open U-shaped end, which is also the preferred location for most scientists. The numbers on the ruler are large and easy to read. The ruler is laser engraved, which can achieve the best durability for all three rulers. The degree of the ruler has reached 0.1mm；

2．Smooth movement The standard brain stereotaxic device adopts a unique three line thread, which can achieve the best position positioning. The positioning process is smooth and linear；

3．adjust By guiding the thread, the operating arm can control the movement in the left, right, and up and down directions, and the front and back control is carried out through a wedge-shaped tenon, which can move 80mm in each direction. The universal joint allows the operator to rotate the gripper up to 90 degrees in the up and down or left and right planes. The improved locking component allows the brain stereotaxic device to lock at any angle without sliding, and also provides excellent 90 degree vertical locking.

4．The operator can move the operating arm or gripper outside the operating area by swinging the joint during the experiment, and then reliably return to the origin when needed；

5．Easy electrophysiological operation The brass sleeve integrated on the operating arm facilitates grounding and can be directly connected to the metal part of the operating arm, even to the clamp；

6．Dual arm model The 51600 dual arm standard series brain stereotaxic device has three directional positioning control arms on both sides, and the * * control arms can be twisted appropriately according to the hand's habits. It is very necessary to use two operating arms for dual injection or simultaneous stimulation and recording；

7．Components included 51600 and 51603 both include a standard base, operating arm, 51631 standard gripper, 51621 rat adapter (nose clamp and 18 ° ear rod)；

routine maintenance

1. Handle with care to avoid damage to the instrument due to collision

2. Choose experimental subjects that do not exceed the applicable range of the instrument

3. After the experiment is completed, the instrument should be cleaned in a timely manner to ensure its performance

Experimental Procedure

1. Firstly, refer to the rat brain stereotaxic map or literature to determine the coordinates of the injection site in the brain, including how many millimeters in front or behind the anterior fontanelle, how many millimeters to the left or right of the midline, and how many millimeters to the depth under the skull.

2. Anesthetize the rats and fix them on a brain stereotaxic device, mainly fixing the nose and ears. After fixation, the rat's head cannot move.

3. Cut off the hair on the top of the rat's head and the surface of the skin, then cut open the skin longitudinally or transversely as needed. Use cotton balls and remove the meninges on the surface of the skull. After the skull dries for a while, find the anterior fontanelle (the cross point near the front). Use the anterior fontanelle as the origin and refer to the positioning coordinates to move the injection needle above the injection site；

4. Use a signature pen to mark the origin point at the corresponding position on the skull, move the injection needle away, and use a dentist to make a small hole at the injection site. If bleeding occurs, stop the bleeding in time. Then move the injection needle back above the injection site, inhale the injection * *, and then move the injection needle to the injection site according to the positioning injection coordinates. Start slowly injecting * *, leave the needle for a while after injection, then slowly remove the injection needle from the brain, suture the skin, inject anti infection into the muscle, and place the animal back in the cage. Pay attention to postoperative insulation.