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Beijing Yuechangxing Technology Co., Ltd
SpectraMax M4 multifunctional enzyme-linked immunosorbent assay (ELISA) reader
NegotiableUpdate on 04/03
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Overview
SpectraMax M4 is a dual grating continuous spectroscopy and multifunctional reading machine launched by Molecular Devices, which has excellent performance comparable to single function reading instruments. Its reading modes include: light absorption (UV visible light), fluorescence intensity (FI), time-resolved fluorescence (TRF), and chemiluminescence (Lum). At the same time, the reading machine also has a built-in colorimetric dish slot.
Product Details
SpectraMax M4 is a patented dual set "filter+grating" continuous spectrum and multifunctional reading machine launched by Molecular Devices, which has excellent performance comparable to single function reading instruments. Its reading modes include light absorption (UV visible), fluorescence intensity (FI), time-resolved fluorescence (TRF), and chemiluminescence (Lum). At the same time, the reading machine also has a built-in colorimetric dish slot, which can measure the reading light absorption, fluorescence intensity, and chemiluminescence separately. The adjustable wavelength monochromator and high-quality reading capability make the SpectraMax M4 multifunctional reader not only suitable for experimental development and scientific research, but also for low to medium flux drug screening.
main features
Patent dual set "filter+grating" optical path design
Patent PATHCHECK optical path sensor technology
Patent automatic gain adjustment Auto PMT function
● Three function colorimetric dish detection (UV visible absorption, fluorescence, chemiluminescence)
application area
The SpectraMax M4 multifunctional microplate reader supports ELISA and FRET of fluorescent proteins in the field of protein detection; DNA/RNA quantification in the field of nucleic acid testing; Phosphatase activity, kinase activity, and protease activity in the field of enzymatic detection applications; Cell migration and cell adhesion in cell function testing; Report genes; Other analyses include fatty acid uptake, neurotransmitter uptake ADME Tox、 Membrane permeability, microbial growth, endotoxin testing, etc.
Experimental examples
Epigenetics involves changes in gene expression levels during cell differentiation or genetic inheritance in offspring, but this change is different from the traditional genetic approach of altering gene sequences. It does not require changes to the gene sequence itself. Histone lysine residue acetylation is crucial for epigenetic regulation of gene transcription. Histone acetylation is believed to occur when a specific protein binds to a known protein specific domain. We used the TR-FRET kit for BRD4 domain 1 and the SpectraMax M4 microplate reader with time-resolved fluorescence resonance energy transfer technology to obtain a more sensitive and reliable high-throughput screening scheme for domain acetylation peptide binding inhibitors for normal cell division and DNA replication detection.
