SpectraMax M2/M2e multifunctional enzyme-linked immunosorbent assay (ELISA) reader

The SpectraMax M2/M2e microplate reader produced by Molecular Devices has multiple reading modes, top and bottom reading (limited to M2e), dual-mode colorimetric dish slots, patented dual set "filter+grating" optical path setup, "PathCheck" technology, and microporous capacity sensors. The reading modes include light absorption mode (UV/VIS absorption) and fluorescence intensity mode (FI). The endpoint method, kinetics, full wavelength scanning, single well scanning, and "PathCheck" sensor enable M2 to perform various homogeneous and heterogeneous microplate detection. SpectraMax M2/M2e can easily optimize and convert very low throughput detection into medium to high throughput analysis, providing you with faster and more accurate detection while saving a lot of reaction reagents. Simply perform simple operations on the software to select between light absorption mode and fluorescence intensity mode.

main features

Patent dual set "filter+grating" optical path design

Patent PATHCHECK optical path sensor technology

Patent automatic gain adjustment Auto PMT function

● Two function colorimetric dish detection (UV visible absorption light, fluorescence)

application area

Mainly used for DNA, RNA and protein quantification, PicoGreen/NanoOrange/Bradford ELISA/EIA detection, phosphatase, kinase, protease detection, microbial growth/MIC IC50/LD50、 Cell viability, cytotoxicity, cell proliferation, fatty acid transfer ADME、 Bacterial endotoxin testing, reporter gene testing, Caspase-3 and protease experiments CatchPoint cAMP/cGMP、 Cytokine detection, with dynamic detection mode, can also be performed for enzymatic detection and dynamic ELISA detection.

Experimental examples

The SpectraMax M2 multifunctional microplate reader has detection functions for fluorescence and light absorption. It adopts a patented dual grating+dual color filter combination to ensure that the optimal excitation and emission positions of the fluorescence signal intensity can be detected in the first time no matter how offset it is. We know that fluorescent proteins play an increasingly important role in monitoring in vivo biological research. The green fluorescent protein (GFP) derived from the Victoria multi tube luminescent jellyfish was the earliest fluorescent protein we applied, but over time, the types of fluorescent proteins we can use have become increasingly diverse, including enhanced variant GFP proteins, fluorescent proteins found in other types of jellyfish, and coral reef proteins. SpectraMax M2 was used for wavelength scanning to illustrate how to optimize wavelengths. In order to detect the optimal excitation wavelength and emission wavelength.