Rapid film transfer instrument

Product Introduction:

HM-ZM rapid film transfer instrument is a fast and efficient WB film transfer equipment. Based on an innovative film transfer method, it combines the advantages of wet transfer and semi dry transfer, and combines the stability of wet transfer and the efficiency of semi dry transfer. It solves the problems of current leakage on the wet transfer side, insufficient buffer solution volume, continuous decrease in buffer solution ion concentration, and rapid attenuation of buffer capacity from the principle of physical force. It can maintain constant pressure and current during film transfer, thus ensuring maximum film transfer efficiency while maintaining film transfer rate. On the basis of membrane transfer, it is combined with Coomassie brilliant blue SDS-PAGE polyacrylamide gel dyeing. Compared with simple manual shaking table dyeing, it is fast and efficient, and can complete dyeing and decoloring in 5 minutes.

Innovative film transfer method:

Traditional semi dry spinning and wet spinning have their own advantages and disadvantages. Proteins below 100KD are suitable for semi dry rotation, and the semi dry rotation rate is fast. Because the current generated by semi dry spinning does not leak and is entirely between the filter papers, the efficiency and speed of spinning are relatively high. However, the disadvantage is that the effective ion concentration decays quickly and may be exhausted in a few minutes. At this time, the large protein has not yet been able to spin, and coupled with the high heat generation, it may burn out. On the contrary, wet conversion results in less effective current, which is not entirely between the filter papers and leaks from the buffer solution side. Therefore, the conversion efficiency is low and the speed is slow. However, the effective ion concentration is high and can be converted for a long time, making it suitable for the conversion of large molecular proteins above 100KD. Unlike traditional semi dry and wet spinning, the M-Blot rapid film transfer instrument uses a semi dry spinning structure to improve the film transfer efficiency. At the same time, it continuously pumps the film transfer liquid into the sandwich structured filter paper to supplement effective ions, so that their concentration is in dynamic equilibrium. Therefore, it can effectively improve the film transfer efficiency while ensuring the speed of film transfer.

Quick protein staining:

After SDS-PAGE polyacrylamide gel electrophoresis, you can directly use M-Blot rapid film transfer instrument to carry out Coomassie brilliant blue dyeing

Thanks to its modular design, sandwich structure, and exclusive fast staining reagents, the color can be completed in just 5 minutes

Dyeing and decolorization do not require a long wait on the shaker.

Feature rich operating software:

New generation upgraded software with diverse functions

The new interface is easier to operate;

Real time voltage and current monitoring;

Run log function to record experimental data throughout the entire process;

Pump control upgrade makes liquid control more effortless;

Upgrade network functions, update programs online, collect and analyze big data on film transfer and staining, and make real-time improvements.

Can store a large number of transfer and staining programs, pre-set, and use more worry free

Pre stored pipeline cleaning program, no need to worry about pipeline cleaning issues

Built in speaker, user-friendly voice reminder upon completion, indicating completion of film transfer and dyeing

Powerful backend data processing, automatically providing corresponding information such as gel concentration and thickness, protein size, name, etc

Film transfer parameters

New revolutionary wet transfer, the buffer solution is new every second during the film transfer process, continuously replenishing effective ions

The fast film transfer process only takes 10-15 minutes;

Save reagents, only 100mL is needed per time;

Open access to reagents and better efficacy of specialized reagents;

Stable constant voltage conversion film, stable current, high reproducibility;

The addition of ice buffer also brings lower temperatures;

Dyeing not only involves transferring the film, but it can also dye;

Simple and convenient full touch screen operation;

Technical Specifications:

Configuration List:

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