New generation Purilad high-throughput protein purifier from Duoning

New generation Purilad high-throughput protein purifier from Duoning

The Purilad protein purification system adopts a high-throughput design with parallel purification of 8 samples, which is convenient, fast, and cost-effective. It is an ideal tool for pharmacology and protein platform research. Purilad can process 8 samples simultaneously and process 48 samples continuously at a time. The system includes an infusion pump, collector, column bracket, and robotic arm. This includes 8 independent valve free metering pumps with a flow rate range of 0.1-30ml/min. When selecting a large capacity *, the flow rate range can be extended to 0.5-60ml/min.

The device software system is convenient and fast, with customizable purification schemes for easy and efficient management. The software provides real-time purification status information, and after purification is completed, it can also help analyze and share results.

Touchscreen operation, convenient and fast, software modular design, including method establishment, operation, permission management, and system settings.

1Technical Specifications

1. Simultaneous processing of sample size: 6-8

2. Continuously process 48 samples

3. Multi combination collection quantity: 96 pieces (collection quantity of 15, 50ml, collection of 48 pieces)

4. Collector type: 96 well plate, 1.5ml EP tube, 15ml centrifuge tube, 50ml centrifuge tube

5. Support column types: 1ml, 5ml, 10ml

6. Surface touch screen operation

7. Number of solvents: 6

8. Can independently open a single channel without affecting the operation of other channels

9. Monitor the sample loading process through sensors

10. The chromatography step is a labor-intensive and time-consuming step in the entire purification process, and as part of the purification process, high-throughput implementation begins with chromatography
11. Automatic collection of samples using UV (280nm) detector (new technology added to next-generation instruments)

IIChromatography steps:

The chromatography step is a labor-intensive and time-consuming step in the entire purification process. Meanwhile, as part of the purification process, high-throughput implementation begins with chromatography.

1. Chromatography: Capture target antibodies from cell culture supernatant

2. Concentration detection: Determine the concentration of antibodies after chromatography

3. gel electrophoresis: observe the concentration through the mobility uniformity of antibodies on the gel

4. Buffer replacement: eliminate the influence of buffer on the activity experiment

5. HPLC: High performance liquid chromatography for detecting antibody purity

IIIApplication scope:

1. Covering the application scope of affinity chromatography, such as Protein A, G and L, GST, Ni column, etc

2. Screening process for stable cell lines (sample size of tens of milliliters or more)

3. Multiple sample preparation of HEK293 and Baculovirus expression system

4. Bioreagent multi sample preparation required for early Functional Assays

5.HTS，HCS， Preparation of samples with high flux requirements and large protein content

4Test data:

Process optimization under different elution conditions

(1) Test 6 buffer conditions, achieve large-scale purification through small-scale purification, and promote stronger purification.

(2) Test the elution curve under the same buffer conditions.

New generation Purilad high-throughput protein purifier from DuoningFor specific details, please consult customer service