French Bio Logic Rapid Mixing Temperature Mutation System

MT Jump Rapid Hybrid Temperature Mutation Systemthe principle of

This instrument is a new typeT-jumpThe instrument changes the temperature by mixing solutions with two different initial temperatures. The final temperature value obtained is calculated based on the initial temperature and mixing ratio of the two solutions.

The device includes3aPeltierComponent, control the temperature changes of the colorimetric cell after mixing the two initial solutions separately.mT-jumpThe attachment needs to be installed inBio-LogicOn the basic flow stop device.

can be passed throughBio-KineSoftware implementation for full control of rapid mixed mutation systems: Through software, users can define reaction mixing ratios and temperature mutation spans.PeltierThe component will automatically adjust the temperature. Automatically accumulating injection samples will * convert the signal-to-noise ratio.

mT-jumpThe system can use a standard colorimetric cell and is used for various forms of spectroscopic measurements such as circular dichroism, ultraviolet absorption, fluorescence anisotropy, etc.

traditionT-jumpSupplement to the system

traditionalT-jumpThe device is used to record the rapid thermal effects of reactions. Usually achieved using fast thermoelectric current pulses or applying more near-infrared pulses to the sample. The time resolution can reach several microseconds, but due to temperature dissipation, dynamic data cannot be recorded for more than a few milliseconds, which limits certain experimental applications. Most temperature mutation devices have a mutation range limited to5℃Internally, cold mutation cannot be achieved yet.

The mT jump rapid mixing temperature mutation system developed by Bio Logic is based on dwell technology and has millisecond level resolution. The mutation amplitude * is determined by the user (up to 40 ℃ mutation span), and both hot and cold bidirectional mutations can be achieved. Cold and hot bidirectional mutations have expanded the field of applied research, such as refolding studies, which do not require the addition of denaturators to proteins for protein folding and refolding experiments. In addition, traditional T-jump systems can only be used for absorption and fluorescence measurements, while mT jump systems are compatible with all measurement modes, including circular dichroism, Fourier transform infrared spectroscopy, fluorescence anisotropy spectroscopy scanning, and so on.

MT Jump Rapid Hybrid Temperature Mutation SystemofSpecification

Temperature Control System Description

lUSBConnection, fully software controlled operation

lTemperature range: 5℃Up to 90 ℃

lTemperature accuracy: 0.01 ℃

lMaximum temperature mutation:/-40 ℃

lTemperature stability in the colorimetric cell after a sudden temperature change:30sInternal changes are less than1%

lIncludes temperature probe

lTemperature stability of syringe 1 and syringe 2 in steady state: less than/-0.2 (after 15 minutes of equilibrium at 80 ℃)

Mixed description

Can be used with allBio-LogicConnected to the dynamic flow stop device

Mixing ratio from1/1to1/4

Sample size for each injection:45μl到220μl

Dead time: determined by colorimetric pool, stop flow mode, and mixing ratio (0.7ms, using SFM-2000, FC-08 colorimetric pool)

can be paired withMOS-200,MOS-450/AF-CD,Connected to other devices such as secondary array tube detectors

Can be paired with Fourier transform infrared colorimetric cell.