The role of genetic library screening technology in drug target discovery in "Xiaobei Talks"~1

1. Research progress on precision drugs and targeted drugs for tumors.
Currently, dozens of targeted therapy drugs have been approved and widely used for the treatment of various malignant tumor diseases. As of 2015, the targeted drug market has exceeded 43 billion US dollars, and its significant growth has attracted more pharmaceutical companies to invest in the research and development of targeted drugs.
But the heterogeneity of tumors is an important characteristic, even for tumors in the same location, the mutation situation of tumors varies greatly among different patients. If testing and targeted medication are not carried out, the effectiveness rate of tumor drugs is only 20%. Therefore, precision medication for tumors is currently a development trend. However, there are still the following difficulties in precision drug therapy for cancer: ⑴ Only a small proportion of tumors have clear therapeutic targets. Most cancer patients cannot detect valuable drug therapy targets. Targeted drugs are prone to developing resistance.
High throughput large-scale genetic screening technology is one of the solutions to the above problems.
Currently, high-throughput screening is an effective means of discovering drug targets or searching for effective therapeutic drugs. High throughput screening can detect tens of millions of samples at the same time, significantly reducing the time required for discovering new drugs and drug targets.
SiRNA and CRISPR technologies are great discoveries in the field of modern life sciences. SiRNA can knock down specific genes
Knockdown effect. CRISPR/Cas9 can also knock out, edit and modify specific genes at the genomic level
Activate. Our lecture mainly introduces the principles of genetic screening library technology. At the same time, it introduces the application of its combination of large-scale cell screening and high-throughput deep sequencing technology in screening and revealing complex signal network regulation, searching for drug targets, and developing combination drug regimens.